Below is only a summary of your post-lab questions. Please see your lab manual for the full questions.
What is the advantage of multiple extractions?
What is the mass of thyme used in the extraction?
Sketch a TLC plate, calculate the Rf values, and label which developing solvent was used.
Did the standards separate?
How does the polarity of the developing solvent affect how far the spots travel?
Based on the Rf values you calculated, which developing solvent would you choose for Part III?
Make a short table summarizing results for all plates from Part III.
Do any extraction samples contain components, which are more polar than thymol and carvacol? How does choice of extraction solvent affect this?
Which of the extraction smaples contain thymol and/or carvacol?
Were you able to separate thymol and carvacol by TLC?
Did your extract contain p-cymene?
Does it make sense that p-cyemene would elute last?
What compounds did you extract with methanol?
Summarize the results of your HPLC analysis with a table.
Graph peak area vs. final concentration of the thymol standard in each flask. Attach a copy of this plot to your Report Sheet.
Calculate Cflask using the method of standard addition.
What is the concentration of the thymol in your extract, the true Cunknown?
Calculate the the mg thymol/g thyme leaf.