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Exp 8: Thymol 1
Home
Introduction
Background
Materials
Safety
Part I
Extractions 1-3
Extraction 4
Part II
Preparing the TLC chamber
Preparing the TLC plate
Visualizing the TLC plate
View TLC Plates part II
100% Heptane MP
25%/75% EtOAC/Heptane MP
50%/50% EtOAC/Heptane MP
75%/25% EtOAC/Heptane MP
Part III
TLC analysis of extract
View TLC data part III
Water Extraction
25%/75% EtOAC/heptane Extraction
Heptane Extraction
Methanol Extraction
Part IV
HPLC of extract
HPLC Instrumentation
HPLC Standards
Sample Data
Group A HPLC analysis
Group B HPLC analysis
Group C HPLC analysis
Group D HPLC analysis
Group E HPLC analysis
Group F HPLC analysis
Part V
Preparing filter disks
Clean up
Results and data analysis
Exp 8: Thymol 1
Home
Introduction
Background
Materials
Safety
Part I
Extractions 1-3
Extraction 4
Part II
Preparing the TLC chamber
Preparing the TLC plate
Visualizing the TLC plate
View TLC Plates part II
100% Heptane MP
25%/75% EtOAC/Heptane MP
50%/50% EtOAC/Heptane MP
75%/25% EtOAC/Heptane MP
Part III
TLC analysis of extract
View TLC data part III
Water Extraction
25%/75% EtOAC/heptane Extraction
Heptane Extraction
Methanol Extraction
Part IV
HPLC of extract
HPLC Instrumentation
HPLC Standards
Sample Data
Group A HPLC analysis
Group B HPLC analysis
Group C HPLC analysis
Group D HPLC analysis
Group E HPLC analysis
Group F HPLC analysis
Part V
Preparing filter disks
Clean up
Results and data analysis
More
Home
Introduction
Background
Materials
Safety
Part I
Extractions 1-3
Extraction 4
Part II
Preparing the TLC chamber
Preparing the TLC plate
Visualizing the TLC plate
View TLC Plates part II
100% Heptane MP
25%/75% EtOAC/Heptane MP
50%/50% EtOAC/Heptane MP
75%/25% EtOAC/Heptane MP
Part III
TLC analysis of extract
View TLC data part III
Water Extraction
25%/75% EtOAC/heptane Extraction
Heptane Extraction
Methanol Extraction
Part IV
HPLC of extract
HPLC Instrumentation
HPLC Standards
Sample Data
Group A HPLC analysis
Group B HPLC analysis
Group C HPLC analysis
Group D HPLC analysis
Group E HPLC analysis
Group F HPLC analysis
Part V
Preparing filter disks
Clean up
Results and data analysis
Part II: TLC Analysis of Standards
The first step in preparing the TLC plate is marking the starting positions of the 3 analytes and the cospot with a pencil on the plate.
What will your plate look like?
Plate 1
Plate 2
Plate 3
Plate 4
Go back to preparing the TLC chamber
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