PCR cleanup

Learning goals

By the end of this lab, students should be able to:

-Describe why we need to clean up our PCR reaction.

-Describe how to clean up a PCR reaction.

Clean PCR product to prepare it for your second PCR reaction.

Safety Information and MSDS

Everything in this lab is safe, and may be discarded in the trash or down the drain.

Reading: How the PCR cleanup works

Read this to learn why you need to clean up your PCR reaction and how you will do it

Assignment: PCR cleanup - how it works

Answer these questions after reading PCR cleanup background.

Protocol: PCR cleanup

Read this to learn how to clean up your PCR product

Read the entire protocol before starting to work

Quiz: PCR cleanup protocol

Complete this assignment after reading the protocol and before starting your cleanup.

Feel free to refer to the protocol as you take the quiz

Assignment: PCR cleanup photos

To show that you carried out the lab, please post photos your procedure and you carrying out the lab. To show that it was you who carried out the lab, make sure you show up in at least one of the pictures, along with your lab setup.