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Learning goals
Learning goals
Run an agarose gel to determine if PCR2 worked
Safety information and MSDS
Safety information and MSDS
Everything in this lab is safe, but they are not made for eating.
Keep them away from food and food preparation equipment.
GelRed/GelGreen binds DNA, but is not absorbed by the skin, so is thought to be safe.
Work on the provided bench paper to absorb any spills.
Centrifuge:
Always balance your centrifuge, and wait until the centrifuge is done spinning before opening the lid.
Disposal and cleaning:
Everything used in this lab may be disposed of in the trash or drain.
Wash hands and clean counter tops when you are done.
The link contains Material Safety Data Sheets (MSDS) for chemicals used in this lab
Protocol: Agarose gel 2
Protocol: Agarose gel 2
Run another agarose gel to see if PCR2 worked. Read this for instructions.
Assignment: Agarose gel 2 post lab
Assignment: Agarose gel 2 post lab
Answer these questions after you run agarose gel 2
Assignment: Agarose gel 2 photos
Assignment: Agarose gel 2 photos
Submit a photo of your gel. Indicate which lanes have PCR1 and PCR2, and which lane has which sample
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