By the end of this lab, you should be able to describe:
the goal of PCR.
the steps in PCR, and what occurs in each step.
Watch this animation to learn how PCR works. Make sure you can describe each step in a PCR reaction, and what happens to the DNA in each step.
Optional: Watch this if you need more help understanding how PCR generates many copies of a particular region of the genome using DNA replication.
Work through this interactive.
Pay close attention to what happens after starting the thermocycler.
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Use the above videos and interactive to answer these questions about how PCR works