Embedded Files
Learning Goals
Learning Goals
You should be able to describe:
the reason for doing PCR in our project.
the ITS genomic region and why we are using it
Video: What is ITS2?
Video: What is ITS2?
Watch this video to learn about the ITS2 genomic region and why we are sequencing this particular region.
Before you can sequence your DNA, you need to PCR amplify (make lots of copies of) a single region of DNA. Then we will sequence this one region of DNA . The sequence from this one region, called ITS2, is sufficient to identify what plant species the DNA is from.
Why don't we just sequence all of the DNA in the sample?
It would cost a ton of money. It would require a ton of computational power to analyze the data, and other parts of the genome aren't as useful for identifying what plant species the DNA is from.
Read this explanation of the PCR reaction that you will set up. Learn about the locus we will amplify and thermocycling conditions
Watch "The ITS2 genomic region and why we are using it" and read "Your PCR Reaction" to answer the questions about our primers and the region we amplified
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