Embedded Files
What will students do?
What will students do?
Your samples will be sequenced on a next generation Illumina sequencer.
There are currently quite a few different sequencing technologies in use, but the Illumina technology is currently the industry standard.
Unfortunately, we cannot run the sequencer ourselves, as only a dedicated technician is allowed to run the sequencer.
However, you should learn how the sequencer works and the chemistry underlying DNA sequencing.
Learning goals
Learning goals
By the end of this unit, you should be able to describe
the molecular details of how Illumina sequencing works
what a flow cell is
what a cluster is and how it is generated
what Bridge PCR is and how it differs from regular PCR
how we determine the DNA sequence of a template from colored dots on the flow cell
Video: Illumina sequencing lecture
Video: Illumina sequencing lecture
Watch this video for a general overview of how Illumina sequencing works and how it is different from the older sequencing technique called Sanger sequencing
Reading: Illumina Sequencing Study guide
Reading: Illumina Sequencing Study guide
Read this for definitions and a very brief overview of Illumina sequencing
Video: Illumina sequencing
Video: Illumina sequencing
Watch this for an explanation of Illumina Sequencing with an intermediate level of detail
Video: Bridge PCR
Video: Bridge PCR
Watch this video for a detailed explanation of the first steps in Illumina sequencing, including addition of sequencing adaptors (you did this with PCR2), and bridge PCR.
If you want to see the video containing questions embedded with playposit, click here. It provides a greatly improved learning experience, but you'll need a playposit licence to assign video quizzes containing playposit questions to your students.
Interactive tutorial: Bridge PCR
Interactive tutorial: Bridge PCR
Work through this interactive to get a better understanding of bridge PCR.
Click here to view at a larger size in a new window
Interactive tutorial: Illumina DNA sequencing
Interactive tutorial: Illumina DNA sequencing
Work through this interactive to get a better understanding of sequencing DNA.
Click here to view at a larger size in a new window
Optional: This is a transcript of the Bridge PCR and Sequencing animations in case you find it helpful
Watch the above videos on Bridge PCR and Illumina DNA sequencing and answer the questions
Assignment: Illumina DNA sequencing
Assignment: Illumina DNA sequencing
Watch the above videos on Illumina DNA sequencing and answer the questions
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