Agarose gel

What will students do in this lab?

Students will run an agarose gel to determine if PCR1 worked.

Learning goals

At the end of this lab, you should be able to

• describe how agarose gels work

• determine the size of a DNA band on an agarose gel

• run an agarose gel

• determine if your PCR reaction worked

Safety information and MSDS

Everything in this lab is safe, but they are not made for eating.

• Keep them away from food and food preparation equipment.

• GelRed/GelGreen binds DNA, but is not absorbed by the skin, so is thought to be safe.

• Work on the provided bench paper to absorb any spills.

Centrifuge:

• Always balance your centrifuge, and wait until the centrifuge is done spinning before opening the lid.

Disposal and cleaning:

• Everything used in this lab may be disposed of in the trash or drain.

• Wash hands and clean counter tops when you are done.

The link contains Material Safety Data Sheets (MSDS) for chemicals used in this lab.