Embedded Files
Antibacterial and Anti-biofilm Effect of Lactuca serriola Extract Against Clinically Isolated Porphyromonas gingivalis and Prevotella intermedia: An in vitro study
Rozhgar R. Sharif * , Aram Mohammed Sha*
* Department of Periodontics, College of Dentistry, University of Sulaimani, Sulaimani, Iraq.
Submitted: August 11, 2022, Accepted: October 13, 2022, Published: December 1, 2022.
DOI: https://doi.org/10.17656/sdj.10160
Objective: To investigate the antibacterial and anti-biofilm effect of Lactuca serriola (LS) extract against clinically isolated Porphyromonas gingivalis (P. gingivalis) and Prevotella intermedia (P. intermedia).
Methods: LS extract was prepared by solvent extraction method. Gas chromatography mass spectrometry (GC-MS) was used to analyze phytochemical components. Plaque samples were obtained from periodontitis patients with probing pocket depth ≥6mm to isolate P. intermedia. Microbiological tests and conventional polymerase chain reaction (PCR) were used to confirm P. intermedia. Antibacterial activities of LS against the confirmed clinical strains (P. intermedia and revised P. gingivalis) were determined by agar well diffusion method. Broth macro dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The anti-biofilm assay of the LS was performed by using tube adhesion method.
Results: Antibacterial activity of LS against clinical isolates revealed that the inhibition zones ranged from 11.75 ±0.95mm to 24.25 ±1.7mm for P. gingivalis and 12.75 ±0.95mm to 25.5 ±1.29mm for P. intermedia. The MICs of LS extract were 212.5mg/mL and 106.2 mg/mL, while the MBCs were 425mg/mL and 212.5mg/mL against P. gingivalis and P. intermedia, respectively. The anti-biofilm of LS extract exhibited strong anti-biofilm activity.
Conclusions: LS extract exhibited bactericidal and anti-biofilm activity against clinically isolated P. gingivalis and P. intermedia in an in vitro study.
Keywords: Antibacterial activity, Anti-biofilm, Lactuca serriola, P. gingivalis, P. intermedia.
References:
1. Ximénez-Fyvie LA, Haffajee AD, Socransky SS. Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis. J Clin Periodontol. 2000;27(9):648-57.
2. Blandino G, Milazzo I, Fazio D, Puglisi S, Pisano M, Speciale A, et al. Antimicrobial susceptibility and β-lactamase production of anaerobic and aerobic bacteria isolated from pus specimens from orofacial infections. J Chemother. 2007;19(5):495- 9.
3. Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. In: Lancet Elsevier. 2005;366(9499):1809-20.
4. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000. 1994;5(1):78-111.
5. Kou Y, Inaba H, Kato T, Tagashira M, Honma D, Kanda T, et al. Inflammatory responses of gingival epithelial cells stimulated with porphyromonas gingivalis vesicles are inhibited by hop-associated polyphenols. J Periodontol. 2008;79(1):174-80.
6. Doğan B, Kipalev AŞ, Ökte E, Sultan N, Asikainen SE. Consistent intrafamilial transmission of actinobacillus actinomycetemcomitans despite clonal diversity. J Periodontol. 2008;79(2):307-15.
7. Lovegrove JM. Dental plaque revisited: bacteria associated with periodontal disease. J N Z Soc Periodontol. 2004;1(87):7-21.
8. Huang D ming, Ling J qi, Fu C hua, Luo H xia, Zhou X dong. Colonization relationship between Porphyromonas gingivalis and Bacteroides forsythus in the infected root canals with chronic apical periodontitis. Shanghai Kou Qiang Yi Xue. 2005;14(5):531-5.
9. Tanabe SI, Bodet C, Grenier D. Peptostreptococcus micros cell wall elicits a pro- inflammatory response in human macrophages. J Endotoxin Res. 2007;13(4):219-226.
10. Saito Y, Fujii R, Nakagawa KI, Kuramitsu HK, Okuda K, Ishihara K. Stimulation of Fusobacterium nucleatum biofilm formation by Porphyromonas gingivalis. Oral Microbiol Immunol. 2008;23(1):1-6.
11. Darveau RP, Hajishengallis G, Curtis MA. Porphyromonas gingivalis as a potential community activist for disease. J Dent Res. 2012;91(9):816-20.
12. Braga RRR, Carvalho MAR, Bruña-Romero O, Teixeira RE, Costa JE, Mendes EN, et al. Quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction. Anaerobe. 2010;16(3):234-9.
13. Bostanci N, Belibasakis GN. Porphyromonas gingivalis: an invasive and evasive opportunistic oral pathogen. FEMS Microbiol Lett. 2012;333(1):1-9.
14. Kim MH, Choi YY, Lee HJ, Lee H, Park J-C, Yang WM. Topical application of herbal formula for the treatment of ligature-induced periodontitis. J Periodontal Implant Sci. 2015;45(4):145-51.
15. RC P. The microbiological case for adjunctive therapy for periodontitis. J Int Acad Periodontol. 2004;6(4):143-9.
16. Puranik M, Harish YR, Anushri M, Yashoda R, Puranik MP. Herbs: A good alternatives to current treatments for oral health problems. Int J Adv Heal Scie IJAHS. 2015;1(12):26-32.
17. Szyszkowska A, Koper J, Szczerba J, Pulawska M, Zajdel D. The use of medicinal plants in dental treatment. Herba Pol. 2010;56(1):97-107.
18. Al-Marzoqi AH, Hussein HJ, Sahi N. Antibacterial activity of the crude phenolic, alkaloid and terpenoid compounds extracts of Lactuca serriola L. on human pathogenic bacteria. Chem Mat Res. 2015;7(1):8-10.
19. Uniyal SK, Singh KN, Jamwal P, Lal B. Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. J Ethnobiol Ethnomed. 2006;2(14):1-8.
20. Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: An expanding role in development.World Bank Publications. 1996;320.
21. Yadava R, Fitoterapia JJ-. New antibacterial triterpenoid saponin from Lactuca scariola. Elsevier. 2008;79(4):245-9.
22. Ahmad F, Khan RA, Rasheed S. Study of analgesic and anti-inflammatory activity from plant extracts of Lactuca scariola and Artemisia absinthium. J Isla Acad Sci.1992;5(2):111-4.
23. Kim DK. Antioxidative components from the aerial parts of Lactuca scariola L. Arch Pharm Res. 2001;24(5):427-30.
24. Sha A M, Taha Garibt B. Antibacterial effect of curcumin against clinically isolated Porphyromonas gingivalis and connective tissue reactions to curcumin gel in the subcutaneous tissue of rats. Biomed Res Int. 2019;2019:1-14.
25. Hama Gharib DS, Salman RF. Feasibility of the crude extracts of Amorphophallus paeoniifolius and Colocasia esculenta as intracanal medicaments in endodontic therapy in comparison to the 940 nm diode laser: An in vitro antimicrobial study. J Dent Sci. 2022; ahead printing.
26. Wunsch CM, Lewis JP. Porphyromonas gingivalis as a model organism for assessing interaction of anaerobic bacteria with host cells. J Vis Exp. 2015;17(106):53408.
27. Abdi-Ali A, Hendiani S, Mohammadi P, Gharavi S. Assessment of biofilm formation and resistance to imipenem and ciprofloxacin among clinical isolates of Acinetobacter baumannii in Tehran. Jundishapur J Microbiol. 2014;7(1):8606.
28. Moghadam ET, Yazdanian M, Tahmasebi E, Tebyanian H, Ranjbar R, Yazdanian A, et al. Current herbal medicine as an alternative treatment in dentistry: In vitro, in vivo and clinical studies. Eur J Pharmacol. 2020;889(1):173665.
29. Jorgensen MG, Slots J. The ins and outs of periodontal antimicrobial therapy. J Calif Dent Assoc. 2002;30(4):297-305.
30. Fine JB, Harper DS, Gordon JM, Hovliaras CA, Charles CH. Short-term microbiological and clinical effects of subgingival irrigation with an antimicrobial mouthrinse. J Periodontol. 1994;65(1):30-6.
31. Zheng CJ, Yoo J-S, Lee T-G, Cho H-Y, Kim Y-H, Kim W-G. Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids.FEBS letters. 2005;579(23):5157-62.
32. Shaaban MT, Ghaly MF, Fahmi SM. Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria. J Basic Microbiol 2021;61(6):557-68.
33. Srinivasan R, Mohankumar R, Kannappan A, Raja VK, Archunan G, Pandian SK, et al. Exploring the anti-quorum sensing and antibiofilm efficacy of Phytol against Serratia marcescens associated acute pyelonephritis infection in Wistar rats. Front Cell Infect Microbiol 2017;7(1):498.
34. Sales-Campos H, Reis de Souza P, Crema Peghini B, Santana da Silva J, Ribeiro Cardoso C. An overview of the modulatory effects of oleic acid in health and disease. Mini Rev Med Chem. 2013;13(2):201-10.
35. Korbecki J, Bajdak-Rusinek K. The effect of palmitic acid on inflammatory response in macrophages: an overview of molecular mechanisms. Inflamm Res. 2019; 68(11):915-32.
36. Al-Marzoqi AH, Hussein HJ, Sahi N. Antibacterial Activity of the Crude Phenolic, Alkaloid and Terpenoid Compounds Extracts of Lactuca serriola L. on Human Pathogenic Bacteria. Chem Mater Rese. 2015;7(1):8-10.
37. Lynch AS, Robertson GT. Bacterial and fungal biofilm infections. Annu Rev Med. 2008;59(1):415-28.
Abstract
© The Authors, published by University of Sulaimani, College of Dentistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
Page updated
Report abuse