156B Week 9

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Optics Design: In Progress

    • Testing of halogen lamp for brightfield

    • Fabricated prototype mount for halogen lamp

  • TIRF Testing: In Progress

    • Continued performing tests to determine appropriate angle, distance, intensity configurations for optimum imaging performance

  • Spectroscopy: In Progress

    • Determine dimensions of apparatus

    • Completed wiring of new line CCD circuit

    • Program to read appropriate data off line CCD in progress

    • Sent inquiry to vendor that manufacturers spectrophotometer arduino shields

    • Assessing whether feasible within time frame of class (spectroscopy is secondary objective)

  • Electronics: In Progress

    • Possible electronics design:

      • Implementation of transistor for device power management

      • Implementation of USB hub

  • Mechanical: In Progress

    • Testing:

      • Implementation of compression springs to keep lead screw engaged

    • Completed first design of slide tray

    • Designing stabilizer for microscope optics

    • Designing outer enclosure for microscope

  • Software: In Progress

    • Development of UI in Java

    • Development of UI in iOS for the iPad

    • Development of high resolution image capture

Accomplishments from Previous Week

• • Fabricated mount for halogen lamp

  • Performed testings of image quality using halogen lamp (brightfield)

  • Purchased remaining components needed for completion of prototype for final demo

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Draft of presentation poster (Kyle, Leo, Reggie, Sean)

  • Final Draft of Report (Kyle, Leo, Reggie, Sean)

  • Slide tray fabrication (Kyle, Leo)

  • Final Draft of Website (Reggie)

  • Complete testing/design of Spectrophotometer (Reggie, Sean)

  • Complete testing for determination of fluorescence dimensions (Reggie, Sean)

  • Fabrication of outer box (Kyle, Leo)

  • Rewiring of electronics using new components (Sean, Leo)

  • Film/edit demo video (Reggie)

  • Photograph final prototype (Reggie)

  • Create graphical components for UI (Reggie)

  • Complete development of UI for iPad (Sean)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments from 5/30/12 Meeting:

    • Quality of image looks strange

      • 3D rather than compressed or flat

      • Could be consequence of using cheaper optics

      • Sponsor would like us to try oil objectives

    • Sponsor would like to have full resolution image capture

    • TIRF requires more testing

      • Sponsor will provide square prism to see if quality improves

    • Would iPad interaction of microscope for demo during finals week

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Comments from 5/24/12 Presentation:

    • Good overview

    • Good CAD early on

    • Would have like to see more details on the optics, especially since the spectroscope seems to have limitations

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • Comments from 5/24/12 Presentation

    • "Your project is probably the most complete of all"

    • "Switching objectives creates a lot of vibration. Maybe slow down that motion."

    • "Cool presentation and pretty solid design. Awesome job."

    • "Maybe create slide slot for slides to come in and out."

    • "Does the casing help for eliminate the vibrations involved?"

Risks and Areas of Concern

• • Properly reading data of line CCD (for spectrophotometer)

  • TIRF Lighting Issues

    • Requires more testing

  • Whether or not to include TIRF and spec in final hardware prototype

Resources or Information Required but not Available

• • Spectrophotometer Arduino shield

    • Sent purchase inquiry to vendor

  • 5MP USB CMOS Sensor

    • Achieved contact with vendor

    • Awaiting confirmation of order and shipment

  • Square Prism for fluorescence testing

    • Waiting on Edgar for fabrication

Schedule

• • Completion of Final Prototype

  • Testing of Prototype Performance

Budget (list amount spent and amount remaining)

• • Amount Spent: $1132.36

  • Amount Remaining: $367.64

Progress on Report and Webpage

• • Final draft of Webpage In Progress

  • Final draft of Report In Progress

  • Uploaded Content onto Multimedia Page