156B Week 2*

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Optics Testing: In Progress

    • Must perform final tests to finalize optics configuration

  • TIRF Testing: On hold

    • Waiting for components to ship

  • Spectroscopy Testing: On hold

    • Waiting for components to ship

  • Electronics: In Progress

    • 2 DOF stepper operation testing complete

    • LED circuit construction on hold

  • Mechanical: In Progress

    • Fabrication of first prototype

  • Software: In Progress

    • Arduino XY stepper code complete

    • Graphical User Interface (GUI) for Ipad application in development

Accomplishments from Previous Week

• • Performed numerous optics experiments

    • Conclusions:

      • Standard tube length yields best image quality

      • Increasing tube length directly affects increase in magnification

        • Shrinking tube length reduces magnification

      • Condenser lens operates the same as eyepiece

        • However is affected by increased chromatic abberations

  • Determined optics configuration with magnification suitable for TIRF

    • 40x objective with eyepiece, condenser lens, and 1.625 inch tube length is sufficient

  • Fabrication of XY translation mechanism

  • Successful operation and control of XY translation mechanism

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Finish fabrication of XYZ translation mechanism (Leonardo, Kyle)

  • Final Optics Testing (Reggie, Sean)

  • Finalize Optics Configuration (Reggie)

  • Fabricate TIRF Test Bed (Kyle, Reggie)

  • Solder and Construct LED circuits (Sean)

  • TIRF Testing - Determine Angle for Proper Fluorescent Imaging (Reggie and Sean)

  • Fabricate Photodiode Mount (Leonardo)

  • Program XYZ for Spectroscopy Scan (Kyle)

  • Spectroscopy Calibration (Leonardo and Sean)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments from 4/9/12 Meeting:

    • Component Suggestions:

      • 5 MP (1.4 micron pixels) CMOS module with lenses

      • Gumstick - miniature Linux box

      • Infinity corrected objectives (may decrease tube length)

    • Desired Features:

      • Be able to switch objectives

      • User should be able to scan slide at 10x, choose focus area, then scan at high magnification

      • Attractive packaging (brushed aluminum or black metal)

      • Solar cell to charge power source

    • Components/Info Needed from Sponsor:

      • Sample slides and reference pictures (to test imaging capabilities for health applications)

      • Microfluidic bead samples (to do TIRF testing)

    • Other:

      • Wants CAD models of semifinal designs

      • Another optics expert to consult with

  • Comments from 4/10/12 Meeting:

    • Desired Features:

      • Spectrometer range - detect 1100nm - 250nm wavelengths

      • Can use 2x photo-diodes with slits

    • Suggested Optics Tests:

      • Use of condenser lens to eliminate tube length

    • For Future:

      • Test and calibrate spectrometer

    • Received Sample Slides:

      • Microfluidic beads, approx. 2 microns

      • Stained bloods cells, approx. 5 microns

  • Actions Taken:

    • Numerous optics tests performed

      • Varying tube length

      • Use of condenser lens

      • Imaging of stained blood and microfluidic beads

      • Theoretical optics calculations for new CMOS sensor (shipping)

    • Designed mechanism to switch between objective lenses

      • Uses a micro servo

    • CAD model in progress

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Comments from 4/5/12 Meeting:

    • Servo Motor vs. Stepper Motor

      • Hobby servo vs. regular servo

    • Over-constrained (high precision, high cost) vs. Exact Constrained (low cost, high performance)

      • Exact constrained design

        • Linear slider: one bearing and one slot

    • Focus on optics research for Week 2

    • Optics trade-off

      • Higher cost optical components (smaller size) vs. Lower cost optical components (larger size)

  • Actions Taken:

    • Implementation of exact constrained design

      • Use of one bearing and one over-sized hole (similar to slot)

    • Numerous optics tests performed

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • N/A

Risks and Areas of Concern

• • Optics for Bright Field Microscopy

Resources or Information Required but not Available

• • 5 MP (1.4 micron pixels) CMOS module with lenses

  • Gumstick - miniature Linux box

  • Infinity corrected objectives (may decrease tube length)

  • Reference pictures (to test imaging capabilities for health applications)

  • Another optics expert to consult with

Schedule

• • Upcoming Milestones

    • Testing Complete

      • Finalized optics configuration

      • Determination of emission angle for TIRF

      • Calibration of Spectroscope

    • First Prototype Complete

Budget (list amount spent and amount remaining)

• • Amount Spent: $446.07

  • Amount Remaining: $1,053.93 

Progress on Report and Webpage

• • Drafting of Content for Home Page

  • Uploaded Content onto Multimedia Page