156B Week 6

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • • Optics Design: In Progress

      • Performed initial tests with 60x objective, 15x eyepiece and new 2MP USB enabled CMOS

      • Captured images that were satisfactory to sponsor

      • Possible implementation of 20x eyepiece for improved magnification and image clarity (brand new, clean optics)

    • TIRF Testing: In Progress

      • Began imaging tests using new CMOS sensor

      • Began performing tests to determine appropriate angle, distance, intensity configurations for optimum imaging performance

    • Spectroscopy: On Hold

      • Determine dimensions of apparatus

      • Possible redesign using line CCD

      • Assessing whether feasible within time frame of class (spectroscopy is secondary objective)

    • Electronics: In Progress

      • Completed wiring and implementation of 2MP USB enabled CMOS

      • Possible electronics design:

        • Implementation of transistor design for switching between stepper motor

        • Implementation of multiplexer for switching between stepper motor

    • Mechanical: In Progress

      • Fabrication of new XYZ translation design completed

      • Testing:

        • Implementation of compression springs to keep lead screw engaged

    • Software: In Progress

      • Photomontaging Code in development

      • Graphical User Interface (GUI) for Mac OS complete

      • Graphical User Interface (GUI) for iPad application in development

Accomplishments from Previous Week

• • Completed fabrication of updated XYZ translation design

  • Completed development of XYZ microscope control Graphical User Interface (GUI) on Mac OS

  • Successfully connected GUI to stepper motor operation via serial communication from laptop computer to Arduino

  • Completed wiring and operational tests of new USB-enabled CMOS module

  • Successfully operated microscope prototype via laptop computer (XYZ control of microscope motion via GUI, live video feed and image capture on laptop)

  • Successfully imaged Blood Smear samples with Brightfield Microscopy and images were approved by two Medical Doctors

  • Completed initial tests for Fluoroscopy (TIRF)

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Reprogram GUI to include options to include speed and delay controls: stepper motor delay time and micro-stepping (Sean)

  • Finish assembly of Objective Rotation Mechanism (Leonardo)

  • Continue TIRF Testing (Reggie, Kyle)

  • Determine Spectroscopy Design (Sean, Leonardo)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments from 5/8/12 Meeting:

    • Likes the images taken by our microscope (blood cells)

    • Sponsor will purchase a 20x eyepiece for possible improvement in optics (current eyepiece has surface scratches which degrade quality)

    • Extremely happy about the "new interaction" as a result of the microscope - no eyepiece, fully automated via computer control, live video feed from computer screen

    • "You have achieved or overachieved the requirements for this project"

    • Possible use of S-mount objective lenses - Sponsor is in contact with manufacturer

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Comments from 5/2/12 Meeting:

    • Discussed briefly project status

    • Instructor would like to see hardware demo

    • Discussed possible camera solutions for the "Leonardo Da Vinci" design problem

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • N/A

Risks and Areas of Concern

• • Focusing Issues with Staph

  • TIRF Lighting Issues

    • Slit width vs. light intensity

  • Ongoing Vibrational Issues with Stage

    • Sensitive to movement in surroundings

Resources or Information Required but not Available

• • Clean Eyepiece

    • Purchased and currently shipping

  • USB Enabled High Resolution CMOS Sensor (5 MP)

    • Having trouble getting a hold of the sales representative

    • Previous sales rep that we were corresponding with no longer works for the company

Schedule

• • Completion of 3rd Microscope Prototype

  • Completion of TIRF and Spectroscopy Testing

Budget (list amount spent and amount remaining)

• • Amount Spent: $978.02

  • Amount Remaining: $521.98

Progress on Report and Webpage

• • First draft of Webpage Complete

  • First draft of Report Complete

  • Uploaded Content onto Multimedia Page