156B Week 3

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Optics Design: Near completion

    • 2-3 objective and eyepiece system with DIN standard tube length

    • Awaiting shipment of CMOS sensor to determine final dimensions

  • TIRF Testing: In Progress

    • Testing of different LEDs: color, brightness, diffusion shape

  • Spectroscopy: In Progress

    • Determine dimensions of apparatus

    • Calibration of sensitivity

  • Electronics: In Progress

    • LED circuit construction

    • Possible design of CMOS electronics (Steve Roberts)

    • Possible implementation of transistor design for switching between stepper motors

  • Mechanical: In Progress

    • Fabrication of first translation prototype completed

  • Software: In Progress

    • Arduino XYZ stepper code complete

    • Photomontaging Code in development

    • Graphical User Interface (GUI) for Mac iOS in development

    • Graphical User Interface (GUI) for iPad application in development

Accomplishments from Previous Week

• • Determined optics configuration with magnification suitable for bright field microscopy

    • 60x objective with eyepiece, and 170mm tube length

    • Imaged staph sample slide

  • Fabricated DIN standard (170mm) microscope tube

  • Fabrication of XYZ translation mechanism

  • Fabrication of adjustable base and microscope tube mount

  • Successful operation of Z translation mechanism

  • Completed electronics wiring and connectors for arduino, stepper motors, and drivers

  • Completed CAD models of preliminary microscope design

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Fabricate TIRF Test Bed (Kyle, Reggie)

  • Complete TIRF Testing (Leo, Kyle, Reggie, Sean)

  • Prepare documentation for electronics circuit design meeting (Sean)

  • Consultation with Steve Roberts about CMOS circuits (Sean)

  • Continue development and coding of User Interface

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments from 4/16/12 Meeting:

    • Believes that optics can be redesigned to be significantly smaller

  • Actions Taken:

    • Contacted Edmund Optics about a custom designed lens system

    • Awaiting a report/calculations from Edmund Optics technician about theoretical optical alignment of two 1.5mm lenses

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Comments from 4/12/12 Meeting:

    • Be more prepared with proper documentation to discuss engineering design problems

  • Actions Taken:

    • Coordinated a make-up meeting with Mike Morris and discussed optics research and results of optics testing

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • N/A

Risks and Areas of Concern

• • Vibration dampening of micrscope tube

  • Electronics debugging - stepper motor drivers are burning out

Resources or Information Required but not Available

• • 5 MP (1.4 micron/pixel) CMOS module with lenses (shipping)

  • Gumstick - miniature Linux box (shipping)

Schedule

• • Upcoming Milestones

    • Testing Complete

      • Determination of emission angle for TIRF

      • Calibration of Spectroscope

    • First Prototype Complete

  • Update Gantt chart.

Budget (list amount spent and amount remaining)

• • Amount Spent: $674.22

  • Amount Remaining: $825.78

Progress on Report and Webpage

• • Drafting of Content for Home Page

  • Uploaded Content onto Multimedia Page