*156B Week 1

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Optics Testing: In Progress

    • Have not determined configuration to achieve target magnification

  • Electronics Testing: Complete

    • Testing of Stepper Motor Operation Complete

  • Mechanical: Complete

    • Fabrication of Translation Test Rig Complete

    • Successful Operation and Control of Translation test Rig Complete

  • Software: In Progress

    • Arduino Stepper Code Complete

    • MAC App Development In Progress

Accomplishments from Previous Week

• • Construction of optics testing rig

    • Webcam straight to objective lens

    • Webcam with objective lens and eyepiece, minimal tube length

    • Webcam with objective lens and eyepiece, standard tube length

  • Successful operation of stepper motor using an arduino and motor driver

  • Fabrication of parts for test stage (lead screw driven linear slider)

  • Successful control of test translation stage

    • Theoretical: 8.33 Microns/step

    • Experimental: 8.30 Microns/step

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Continue testing optical configuration to achieve desired magnification (approx. 60 pixels per micron)

    • Variables:

      • Objective power

      • Eyepiece power

      • Tube length

      • Image sensor size and maximum resolution

  • Test

  • Begin testing of TIRF imaging

    • Determine optimal angle using prism

  • Begin testing of spectroscopy

    • Obtain diffraction grating/prism

    • Determine apparatus dimensions/distances

      • Light Source

      • Sample

      • Slit

      • Grating/Prism

      • Photodiode/Line CCD

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments (4/2/12, in person)

    • Updated Requirements:

      • Magnification/Resolution: 60 pixels per micron

      • Possible use of solar cell

    • Design Suggestions/Possible Solutions:

      • Eliminate tube length and eyepiece and test webcam directly to objective

      • Photo-montaging using highest magnification instead of two difference objective lenses

  • Actions Taken:

    • Made optical tests and was not able to resolve image with suggested configuration (webcam directly to objective)

    • Other Tests

      • Webcam with objective lens and eyepiece, minimal tube length - image resolved, less than desired magnification

      • Webcam with objective lens and eyepiece, standard tube length - image resolved, still less than desired magnification

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • N/A (Spring Break)

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • Comments from Risk Reduction Presentation (3/20/11, written comments)

    • Things for future presentations:

      • Professional attire

      • Define portable - clarify dimensions of microscope

      • State how device will be powered

      • Use of laser pointer to point out key points on figures

    • Design Suggestions:

      • Implement higher quality lenses for use with current hardware

      • Precautions for damage of microscope during transport/shipping

  • Received catalog of digital microscopes from another student (4/3/12, in person)

Risks and Areas of Concern

• • Optics:

    • Still unable to achieve target magnification with current optics and image sensor components

    • Desired magnification: Approximately 60 pixels per 1 microns

Resources or Information Required but not Available

• • Higher powered objective lenses and eyepieces

  • Columnating lens - eliminate tube length and eyepiece (as suggested by Edgar)

  • Prism - for TIRF testing

Schedule

• • Upcoming Milestones

    • Complete Test

Budget (list amount spent and amount remaining)

• • Amount Spent: $334.89

  • Amount Remaining: $1,165.11 

Progress on Report and Webpage

• • Risk Reduction Report Uploaded

  • Started Uploading Photos to Multimedia Page