*156A Finals Week

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Risk Reduction Tests: Completed

    • Purchased USB Microscope

    • Hands on experiments of Bright Field, Traditional Fluoroscopy, and TIRF

  • Component Research: In Progress

  • Design: In Progress

Accomplishments from Previous Week

• • Meeting with Steve Roberts about electronics requirements of microscope

    • Arduino

    • Stepper Motor Drivers and Code

    • Power Source Solutions

    • Wifi Shield

  • Meeting with Edgar Guiterrez

    • Performed hands on experiments of traditional Fluoroscopy and TIRF

  • Testing of the off-the-shelf USB Microscope

    • Calibration of microscope's actual magnification

    • Successful TIRF trial using a prism and a while LED

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Continue Component Research

  • Individual Component Analysis

    • Kyle: Stepper Motors and Drivers

    • Leonardo: Motor Coupling and Lead Screws for XYZ Translation

    • Reggie: Optics

    • Sean: Microcontrollers

  • Order Mechanical Components

    • Ground Rod

    • M2 Threaded Screw

  • Order Electrical Components

    • Microcontroller: Arduino Pro Mini

    • Motor Driver: A4988

    • Wifi Shield for Arduino

    • HD Webcam

    • LEDs

  • Order optical components

    • 10x Objective

    • Eyepiece

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Comments:

    • "You guys rock. Great work." (3/14/2012, via email after video conference)

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Comments:

    • "Team members must gain hands on familiarity with the imaging technology and techniques. Perform experiments and try to find out what makes good images and bad images" (3/8/2012, in person)

    • "Ask [Edgar Guiterrez] questions" (3/15/2012, in person)

  • Actions Taken:

    • Visited Edgar's lab and performed hands on experiments of Fluoroscopy and TIRF and determined required conditions for good imaging

    • Obtained sample slides, microfluidic beads, LED light, and other materials to perform further testing on our own

    • Obtained sample target images as a benchmark to compare our microscope's images

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • None

Risks and Areas of Concern

• • USB Microscope did not achieve 200x magnification as manufacturer stated

    • Redesign of optics system may increase overall size of microscope

Resources or Information Required but not Available

• • Parts must still be ordered in order to continue further testing, design, and prototyping

Schedule

• • Finalize Design for First Protoype

  • Update Gantt chart.

Budget (list amount spent and amount remaining)

• • Recent Purchases:

    • USB Microscope, $62.95

  • Amount Remaining: $937.05

Progress on Report and Webpage

• • Webpage Setup Complete