156B Week 7

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • • Optics Design: In Progress

      • Fabricated adapter for newly purchased 20x eyepiece

      • Performed image testing of 25x eyepiece and high quality 10x Nikon eyepiece

    • TIRF Testing: In Progress

      • Continued performing tests to determine appropriate angle, distance, intensity configurations for optimum imaging performance

    • Spectroscopy: In Progress

      • Determine dimensions of apparatus

      • Began redesign of spectrophotometer using line CCD

      • Completed wiring of line CCD circuit

      • Program to read appropriate data off line CCD in progress

      • Sent inquiry to vendor that manufacturers spectrophotometer arduino shields

      • Assessing whether feasible within time frame of class (spectroscopy is secondary objective)

    • Electronics: In Progress

      • Possible electronics design:

        • Implementation of transistor design for switching between stepper motor

        • Implementation of multiplexer for switching between stepper motor

    • Mechanical: In Progress

      • Fabrication of higher precision CMOS plate complete

      • Testing:

        • Implementation of compression springs to keep lead screw engaged

      • Performed risk reduction test of slide tray design

    • Software: In Progress

      • Photomontaging Code in development

      • Updated Graphical User Interface (GUI) for Mac OS complete

      • Graphical User Interface (GUI) for iPad application in development

Accomplishments from Previous Week

• • Refabricated new CMOS plate

  • Fabricated new slotted bushings with new tolerances +/- 0.002

  • Completed wiring for line CCD circuit

  • Received new optics (eyepieces) from sponsor

  • Fabricated initial risk reduction test for slide tray

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • 2nd Draft of Report (Kyle, Leo, Reggie, Sean)

  • Slide tray design (Kyle, Leo)

  • 2nd Draft of Website (Reggie)

  • Complete testing/design of Spectrophotometer (Reggie, Sean)

  • Complete testing for determination of fluorescence dimensions (Reggie, Sean)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Notes from 5/16/12 Meeting:

    • Received optical components from sponsor:

      • Two 20x eyepieces

      • 25x eyepiece

      • High quality brand name 10x eyepiece

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Presentation Comments from 5/10/12:

    • Good hardware progress

    • Good presentation

    • To reduce vibrations consider ramping up speed and ramping down

    • Consider delrin or other plastic for fabrication of linear actuator

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • Presentation Comments from 5/10/12:

    • User Interface Suggestions

      • Buttons move the motor when momentarily pressed rather than on/off motor controls

    • Mechanical Suggestions

      • High friction setup so that kinetic and static friction are almost equivalent = no jump as you overcome static friction

      • Pads at the base of microscope to reduce vibrations

      • No lash flanges for lead screw to help with backlash

Risks and Areas of Concern

• • Properly reading data of line CCD (for spectrophotometer)

  • TIRF Lighting Issues

    • Requires more testing

  • Optical alignment

Resources or Information Required but not Available

• • Spectrophotometer Arduino shield

    • Sent purchase inquiry to vendor

  • 5MP USB CMOS Sensor

    • Awaiting response from vendor

Schedule

• • Completion of 3rd Microscope Prototype

  • Completion of TIRF and Spectroscopy Testing

Budget (list amount spent and amount remaining)

• • Amount Spent: $988.02

  • Amount Remaining: $511.98

Progress on Report and Webpage

• • Second draft of Webpage In Progress

  • Second draft of Report In Progress

  • Uploaded Content onto Multimedia Page