Chamber

Make the slide

First of all, we need to make a chamber. We use this chamber to hold the fluid with Holliday junction. A chamber consist of two pieces of glass, separated by double sided tape. Since you need to flush the channel multiple times, you use a glass slide with holes drilled into it. The coverslip is cleaned with KOH before you receive it.

- Get a microscope slide with holes

- Lay the slide on a tissue, so it won’t get damaged

- Now cut multiple pieces of double sided tape, and lay them along short side of the substrate, in between the holes

- Take a cover glass from the box you get from your TA

- Place the cover glass in the middle of the slide

- Cut the double sided tape along the cover glass and remove it.

- Seal the chamber with epoxy

You now have created multiple little chambers between the slide and the cover glass. This chamber will hold around 100 microliter.