Details of 2023 Reference Genome Annotation Update R64.4

R64.4 Annotation update summary

The S. cerevisiae strain S288C reference genome annotation was updated in 2023. The new genome annotation is release R64.4.1, dated 2023-08-23. Note that the underlying genome sequence itself was not altered in any way. 

This annotation update included (details in table below):

• new uORFs for 3 ORFs:

  • FPS1/YLL043W

  • ACC1/YNR016C

  • HOL1/YNR055C

• 8 new ncRNAs:

  • SUT035/YNCC0015W

  • SUT053/YNCD0033W

  • SUT468/YNCD0034C

  • SUT532/YNCG0047C

  • SUT125/YNCG0048W

  • SUT126/YNCG0049W

  • SUT390/YNCP0025W

  • SUT418/YNCP0026W

• 3 ORFs demoted from 'Uncharacterized' to 'Dubious' based on request from NCBI because they overlap tRNAs:

  • YDR278C

  • YOL013W-A

  • YPR108W-A

R64.4 Annotation update detail

Details of 2021 Reference Genome Annotation Update R64.3

The S. cerevisiae strain S288C reference genome annotation was updated in its first major update since 2014. The new genome annotation is release R64.3.1, dated 2021-04-21. Note that the underlying genome sequence itself was not altered in any way.

R64.3 Annotation update summary

This annotation update included (details in table below):

• 7 new ORFs: OTO1/YGR227C-A, YHR052C-B, YHR054C-B, YJR107C-A, YKL104W-A, YLR379W-A, YMR008C-A

• 5 new ncRNAs: GAL10-ncRNA, TBRT/XUT_2F-154, SUT169, PHO84 lncRNA, GAL4 lncRNA

• 2 new uORFs for ROK1/YGL171W

• 1 new recombination enhancer: RE

• 1 new LTR: YELWdelta27

• 3 ORFs with shifted translation starts: HPA3/YEL066W, YJR012C, LTO1/YNL260C

• 1 ORF with shifted translation stop plus new intron: LDO45/YMR147W

• Changed feature_type (and SO_term) for non-transcribed spacers: NTS1-2, NTS2-1, NTS2-2

• New systematic nomenclature system for entire annotated complement of ncRNAs

Various sequence and annotation files are available on SGD's Downloads site.

R64.3 Annotation update detail

Details of 2011 Reference Sequence Update

The S. cerevisiae strain S288C reference genome sequence was updated in its first major complete update since 1996. The new genome sequence (R64.1.1, 2011-02-03) was compared to the previous version (R63.1.1, 2010-01-05) and corrected accordingly. The sequences of all 16 nuclear chromosomes were updated, resulting in amino acid sequence changes to the 194 proteins listed below. Note that in addition to these, silent changes were made in 42 ORFs. For more information and a complete set of sequence changes, please see:

Engel SR, et al. (2014) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda) Mar 20;4(3):389-98.
SGD paper | PubMed | Full-Text