Crotalus horridus
The Gadsden flag, featuring a timber rattlesnake and the motto "Don't Tread on Me," has evolved significantly in its meaning and use since its creation during the American Revolution. Initially intended as a symbol for the Continental Navy, its representation of a rattlesnake quietly lying in wait, ready to strike if provoked, was mirrored the colonies' attitude towards British opression.
Over time, the flag's meaning shifted as it was adopted by various political and social movements. In recent years, it has been prominently displayed at Tea Party rallies, where it symbolizes opposition to government overreach and a call for personal freedom and adherence to the foundational principles of the United States. The Gadsden flag is often flown in contemporary political protest for lower taxes, less regulation of business operations and of handguns, and resistance to the Affordable Care Act.
After a recent rise of conservative right-wing populism the Gadsden flag is can be associated with both liberty and racist undertones. - Old Flags, New Meanings, Gadsden flag
Gadsden Flag