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Applied Photophysics SX 20 Stopped Flow
Applied Photophysics SX 20 Stopped Flow
Stopped Flow spectroscopy used to measure fast reaction kinetics at milliseconds to seconds time scale. Two solutions carrying reaction partners are mixed rapidly in a flow cell and the flow stopped. Cell illuminated and emitted light detected as a function of time by a photodiode array.
Stopped Flow spectroscopy used to measure fast reaction kinetics at milliseconds to seconds time scale. Two solutions carrying reaction partners are mixed rapidly in a flow cell and the flow stopped. Cell illuminated and emitted light detected as a function of time by a photodiode array.
Applications:
Applications:
• Enzyme kinetics
• Enzyme kinetics
• Binding kinetics
• Binding kinetics
• Redox kinetics
• Redox kinetics
• Equilibrium titration
• Equilibrium titration
PickoScreen 2 (PS02)
PickoScreen 2 (PS02)
• Determination of the diffusion properties of fluorophores or fluorescently labelled molecules (ligands, proteins, DNAs) via Fluorescence Correlation – and Cross Correlation Spectroscopy (FCS and FCCS).
• Determination of the diffusion properties of fluorophores or fluorescently labelled molecules (ligands, proteins, DNAs) via Fluorescence Correlation – and Cross Correlation Spectroscopy (FCS and FCCS).
• Determination of brightness of fluorophores and fluorescently labelled molecules using Fluorescence Intensity Distribuation Analysis (FIDA).
• Determination of brightness of fluorophores and fluorescently labelled molecules using Fluorescence Intensity Distribuation Analysis (FIDA).
• Determination of fluorescence anisotropy of fluorophores and fluorescently labelled molecules using 2D-FIDA-r, i. e. obtaining binding curve.
• Determination of fluorescence anisotropy of fluorophores and fluorescently labelled molecules using 2D-FIDA-r, i. e. obtaining binding curve.
• Determination of structures of proteins via Foerster Resonance Energy Transfer (FRET).
• Determination of structures of proteins via Foerster Resonance Energy Transfer (FRET).
• Confocal scanning via scanning stage.
• Confocal scanning via scanning stage.
• Bead picking.
• Bead picking.
• Lasers: 488nm, 563nm, 640nm and 2 APDs.
• Lasers: 488nm, 563nm, 640nm and 2 APDs.
PickoScreen 4 (PS04)
PickoScreen 4 (PS04)
• Determination of the diffusion properties of fluorophores or fluorescently labelled molecules (ligands, proteins, DNAs) via Fluorescence Correlation – and Cross Correlation Spectroscopy (FCS and FCCS).
• Determination of the diffusion properties of fluorophores or fluorescently labelled molecules (ligands, proteins, DNAs) via Fluorescence Correlation – and Cross Correlation Spectroscopy (FCS and FCCS).
• Determination of brightness of fluorophores and fluorescently labelled molecules using Fluorescence Intensity Distribuation Analysis (FIDA).
• Determination of brightness of fluorophores and fluorescently labelled molecules using Fluorescence Intensity Distribuation Analysis (FIDA).
• Determination of fluorescence anisotropy of fluorophores and fluorescently labelled molecules using 2D-FIDA-r, i. e. obtaining binding curves.
• Determination of fluorescence anisotropy of fluorophores and fluorescently labelled molecules using 2D-FIDA-r, i. e. obtaining binding curves.
• Probing structures of proteins via Foerster Resonance Energy Transfer (FRET).
• Probing structures of proteins via Foerster Resonance Energy Transfer (FRET).
• Confocal scanning via spinning disks.
• Confocal scanning via spinning disks.
• Bead picking.
• Bead picking.
• Lasers: 488nm, 563nm, 640nm and 2 APDs, and 488nm and 640nm for spinning disks imaging.
• Lasers: 488nm, 563nm, 640nm and 2 APDs, and 488nm and 640nm for spinning disks imaging.
Insight Reader (PS05)
Insight Reader (PS05)
• Screening via fluorescence lifetime change upon binding of fluorescently tagged compounds to target proteins via Confocal fluorescence Lifetime aAnalysis (cFLA).
• Screening via fluorescence lifetime change upon binding of fluorescently tagged compounds to target proteins via Confocal fluorescence Lifetime aAnalysis (cFLA).
• Determination of lifetime within 1 min.
• Determination of lifetime within 1 min.
• Pulsed 633nm laser.
• Pulsed 633nm laser.
• Other Applications: FCS, FCCS, FRET, FIDA, 2D-FIDA-r as above.
• Other Applications: FCS, FCCS, FRET, FIDA, 2D-FIDA-r as above.
• Lasers: 488nm and 563nm.
• Lasers: 488nm and 563nm.
Opera
Opera
• Fast imaging using spinning disk technology for cellular screens and live cell imaging.
• Fast imaging using spinning disk technology for cellular screens and live cell imaging.
• 4 Lasers: 445nm, 488nm, 561nm, 640nm. UV and 670nm LED for bright-field, 4 cameras.
• 4 Lasers: 445nm, 488nm, 561nm, 640nm. UV and 670nm LED for bright-field, 4 cameras.
• Robot arm and plate stacker for automated loading and unloading of plates.
• Robot arm and plate stacker for automated loading and unloading of plates.
• Powerful image analysis software allows ease of handling high content screens.
• Powerful image analysis software allows ease of handling high content screens.
LEAP
LEAP
• Unique combination of Imaging, Cell Processing (Transfection by optoinjection and cell ablation).
• Unique combination of Imaging, Cell Processing (Transfection by optoinjection and cell ablation).
• Cell purification via laser ablation to remove unwanted cells from population.
• Cell purification via laser ablation to remove unwanted cells from population.
• Delivery of non-permeable compounds into cells via optoinjection.
• Delivery of non-permeable compounds into cells via optoinjection.
Copas
Copas
• COPAS (Union Biometrica) is a flow cytometry system for high-throughput analysis and sorting of objects ranging in size from 20-1500 µm.
• COPAS (Union Biometrica) is a flow cytometry system for high-throughput analysis and sorting of objects ranging in size from 20-1500 µm.
• It can be used to sort large amount of hit-beads or small multicellular organisms (C. elegans, zebrafish embryos, etc).
• It can be used to sort large amount of hit-beads or small multicellular organisms (C. elegans, zebrafish embryos, etc).
Horiba Flurolog tau3
Horiba Flurolog tau3
• Spectrofluorometer for determination of fluorescence spectra of fluorophores and fluorescently labelled molecules.
• Spectrofluorometer for determination of fluorescence spectra of fluorophores and fluorescently labelled molecules.
• Determination of anisotropy (rotational diffusion time) and binding curve.
• Determination of anisotropy (rotational diffusion time) and binding curve.
• Determination of fluorescence lifetime of fluorophores.
• Determination of fluorescence lifetime of fluorophores.
• 384 plate sample reader.
• 384 plate sample reader.
Chromatography Instrumentation
Chromatography Instrumentation
The Auer Lab operates several High-Pressure-Liquid-Chromatography (HPLC) instruments. Two Agilent 1100 series analytical Reversed-Phase (RP)-HPLC systems are used for dyes or fluorescently labelled samples, and for non-fluorescent or label-free samples. These two systems are routinely used for running quality control of small chemical compounds, peptides, and proteins. There is also an Agilent 1100 series preparative RP-HPLC used for the medium scale purification of mostly in-house synthesised compounds. We have a wide range of columns in used on the three Agilent instruments, suitable for most types of samples (small molecules, peptides, proteins using RP18, RP8 and RP4 columns representing different lengths of hydrophobic chains). Instrument number four is a Dionex (Thermo) analytical Size Exclusion Chromatography (SEC)-HPLC which is used for the quality control of expressed and purified proteins testing their oligomerization or aggregation and folding state. For purification of expressed target proteins, we use an ÄKTA FPLC system. We apply a range of columns ranging from gel filtration to ion-exchange columns on the ÄKTA system.
The Auer Lab operates several High-Pressure-Liquid-Chromatography (HPLC) instruments. Two Agilent 1100 series analytical Reversed-Phase (RP)-HPLC systems are used for dyes or fluorescently labelled samples, and for non-fluorescent or label-free samples. These two systems are routinely used for running quality control of small chemical compounds, peptides, and proteins. There is also an Agilent 1100 series preparative RP-HPLC used for the medium scale purification of mostly in-house synthesised compounds. We have a wide range of columns in used on the three Agilent instruments, suitable for most types of samples (small molecules, peptides, proteins using RP18, RP8 and RP4 columns representing different lengths of hydrophobic chains). Instrument number four is a Dionex (Thermo) analytical Size Exclusion Chromatography (SEC)-HPLC which is used for the quality control of expressed and purified proteins testing their oligomerization or aggregation and folding state. For purification of expressed target proteins, we use an ÄKTA FPLC system. We apply a range of columns ranging from gel filtration to ion-exchange columns on the ÄKTA system.
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