7^th INTERNATIONAL MEETING

STEROIDS AND NERVOUS SYSTEM

TORINO – Orbassano - February 16-20, 2013

February 16 (Dept. Neuroscience - Anatomical Institute, Torino)

Satellite Symposium

ALLOPREGNALONONE: STATE OF ART

Organizers: R.D. Brinton (USA), R.C. Melcangi (Italy) and G.C. Panzica (Italy)

• Melcangi R.C. (Italy), Giatti S., Calabrese D., Mitro N., Viviani B., Garcia-Segura L.M., Caruso D. - Neuroactive steroid levels in physiological and pathological status: focus on progesterone metabolites.

• Lambert J.J. (UK), Brown A.R., Gunn B.G., Belelli D. - Neurosteroids: Local modulators of neuronal GABA-A receptors

• Darbra S., Mòdol L., Llidó A., Casas K., Navarro X., Vallée M., Pallarès M. (Spain) - Neonatal allopregnanolone levels alteration: effects on behaviour and role of the hippocampus

• Nothdurfter C. (Germany), Schüle C., Rupprecht R. - The role of allopregnanolone in depression and anxiety

• Backstrom T. (Swedeen), Nyberg S., Strömberg J., Ragagnin G., Timby E., vanWingen G., Ossewaarde L., Bixo M., Savic I. - Allopregnanolone and mood disorders

• Schumacher M. (France), Liu A., Liere P., Sitruk-Ware R1., Ghoumari A., Guennoun, R. - Allopregnanolone and progesterone receptors: their respective significance in myelination and neuron viability

• Brinton R.D. (USA) Allopregnanolone as a Regenerative Therapeutic for Alzheimer's Disease: Current Status and Future Promise

• Patte-Mensah C., Meyer L., Taleb O., Mensah-Nyagan A.G. (France) - Therapeutic potential of neurosteroid allopregnanolone for the treatment of neuropathic pain

• Hirst J.J. (Australia), Bennett G.A., Kelleher M.A, Walker D.W., Palliser H.K - Neurosteroid disruption leads to adverse outcomes following compromised pregnancy and premature birth.

• Brunton P.J. (UK), Donadi M.V., Yao S.T., Greenwood M., Paris J.J., Frye C.A., Murphy D., Russell J.A. - Sex-dependent overwriting of prenatally programmed stress responses with neuroactive steroids

February 16

18.00 HISTORICAL LECTURE

Balthazart J. (Belgium) Steroid action in the brain: a historical viewpoint from Torino

(Chair: García-Segura L.M.)

February 17 - Department of Clinical and Biological Sciences, Hospital San Luigi, Regione Gonzole 10, Orbassano (Turin).

NEUROACTIVE STEROIDS AND PSYCHIATRIC DISORDERS

Chair: T. Backstrom (Sweeden) & M.A. Riva (Italy)

• Biagini G. (Italy), Rustichelli C., Curia G., Lucchi C., Pugnaghi M., Meletti S., Avoli M. - Neurosteroids and epileptogenesis

• Bortolato M. (USA) - Role of 5alpha-reductase in behavioral regulation: relevance to schizophrenia and other neuropsychiatric disorders

• Frye C.A. (USA) - Pregnane xenobiotic receptors and membrane progestin receptors, sources and targets, for neurosteroid-mediated motivated behavior

• Iacobas D.A., Iacobas S., Chachua T., Sidyelyeva G., Goletiani C., Velíšková J., Velíšek L. (USA) - Modification of glutamatergic and GABAergic synapse gene fabric by prenatal corticosteroids

• Dalla C. (Greece), Kokras N., Pastromas N., Kafetzopoulos V., Balthazart J., Cornil C. A., Papadopoulou-Daifoti Z. - Blockade of neuroestrogens enhances depressive behavior of female, but not male rats in the forced swim test

• Wong P., Chang C.C.R., Marx C.E., Caron M.G., Wetsel W.C., Zhang X. (Singapore) - Pregnenolone rescues schizophrenia-like behavior in dopamine transporter knockout mice

PLENARY LECTURE

Chair: A.G. Mensah-Nyagan (France)

Belelli D. (UK), Gunn B.G., Cunningham L., Lambert J.J. - Neurosteroids and GABA(A) Receptor Interactions: A Focus on Stress.

NEUROPROTECTIVE EFFECTSChair: L.M. Garcia-Segura (Spain) & B. Viviani (Italy)

• De Nicola A.F. (Argentina), Gonzalez-Deniselle M.C., Garay L., Meyer M., Gargiulo-Monachelli G., Guennoun R., Schumacher M., Carreras M.C., Poderoso J.J. - Progesterone protective effect in neurodegeneration and neuroinflammation

• Gravanis A. (Greece) - Cross-talk of neurosteroids and neurotrophins in controlling neuronal survival

• Sohrabji F. (USA), Selvamani A., Miranda R.C. - Estrogen-IGF-microRNA interactions in stroke neuroprotection.

• Saldanha C.J. (USA), Duncan K.A. - Cell‐specific provision of estrogens in the songbird brain

• Yadid G. (Israel), Croitoru O., Sudai E., Ben-Tzion M. , Gispan I- Dehydroepiandrosterone reverse cocaine-induced decrease in hippocampus neurogenesis and attenuate reinstatement

• Ghandour M.S. (France), Hussain R., Ghoumari M.A., Schumacher M. - Androgen receptor is a therapeutic target for myelin repair

IN MEMORY OF ROBERT PURDY - G. Biggio (Italy), M. Vallée (France)

(chair R.C. Melcangi)

February 18

MECHANISMS OF RAPID REGULATION OF BEHAVIOR BY ESTROGENS

Chair: C.A. Cornil (Belgium) & G.C. Panzica (Italy)

• Deepak P. Srivastava D. (UK) - Molecular mechanisms underlying rapid estrogenic-modulation of cortical connectivity

• Remage-Healey L. (USA) - Rapid synthesis and action of estrogens within forebrain circuits in the context of behavior

• Cornil C.A. (Belgium) - Rapid control of reproductive behavior by locally synthesized estrogens-focus on aromatase

• Phan A., Ervin K., Gabor C.S., Choleris E. (Canada) - Rapid estrogenic regulation of social and non-social learning

• Filová B., Malinová M., Bábíčková J., Tóthová Ľ., Ostatníková D., Celec P., Hodosy J. (Slovakia) - Non-genomic effect of steroid hormones on behavior

• Seredynski A.L. (Belgium), Balthazart J., Ball G.F., Cornil C.A - Acute effects of putative membrane estrogen receptors agonists and antagonists on male sexual motivation In Japanese Quail

PLENARY LECTURE

Galea L.A.M. (Canada) - Why sex and sex hormones matter for brain health: Steroid hormone modulation of cognition, emotion and hippocampal neuroplasticity

Chair: M.M. McCarthy (USA)

AGING

Chair: - L.M. Garcia-Segura (Spain) & M. Schumacher (France)

• Urbanski H.F. (USA) - Regulation of neurosteroidogenesis in the nonhuman primate

• Gibson G. (UK) - Progesterone and ischemia in ageing mice

• Hogervorst E. (UK) Effects of gonadal hormones on cognitive behavior in aged men and women

• Morrison J.H. (USA), Hao J., Hara Y., Dumitriu D., Wang A.C.J., Bailey M.E., Bloss E.B., Ohm D.T., Janssen W.G.M., Yuk F., Puri R., Baxter M.G., Rapp P.R. - Synaptic correlates of cognitive performance: Implications for cognitive aging and menopause

February 19

EPIGENETICS

Chair: M.M. McCarthy (Usa) & N. Mitro (Italy)

• Frick K.M. (USA) - Epigenetic regulation of estrogen-induced memory enhancement

• Bale T.L. (USA) - Prenatal stress reprogramming of offspring stress dysregulation: miRNA and placental contributions

• Auger A.P. (USA) - Epigenetic organization of the juvenile social brain and sex-biased vulnerability to mental health disorders

• McCarthy M.M. (USA), Nugent, B.M. Epigenetics of sex differentiation

ROUND TABLE

Effects of Finasteride treatment on the nervous system

Chair: R.C. Melcangi (Italy) & G.C. Panzica (Italy)

Speakers: Worthington A. (USA), Traish A.M. (USA), Melcangi R.C. (Italy), Bortolato M. (USA),

Walf A.A. (USA) & Frye C.A. (USA)

February 20

ESTROGEN-REGULATED SYNAPTIC CONNECTIVITY: NEW APPROACHES OF SIGNALING

Chair: J. Balthazart (Belgium) & G.M. Rune (Germany)

• Kramár E. (USA) - Estrogens rapid influence on synaptic plasticity: interactions with integrins and actin cytoskeleton

• Micevych P.E. (USA) - Membrane estrogen actions: mGluRs, spines and sexual receptivity.

• Rune G.M. (Germany) Phosphorylation of aromatase by calcium-induced calcium release: implications for synaptic connectivity.

• Abraham I.M. (N. Zealand) - Single molecule analysis of non-classical estradiol action in live neurons.

• Zhang L. (Mexico), Hernández V. S. - Ovariectomy down-regulates the hypothalamic vasopressin PVN and SON volume, AVP/Aromatase co-expressing cell numbers and AVP fibres In amydgala and hippocampus: Its impact on anxiety and spatial learning

PLENARY LECTURE

Chair: G.C. Panzica (Italy)

Bourguignon J.-P. (Belgium), Franssen D., Gérard A., Lebrethon M.-C., Rasier G. and Parent A.-S. - Neuroendocrine disruption across reproduction and energy balance.

GENOMIC AND NON-GENOMIC EFFECTS OF NEUROACTIVE STEROIDS: MECHANISMS OF ACTION

Chair: J.J. Lambert (UK) & R.C. Melcangi (Italy)

• Marin R. (Spain), Casañas V., Pérez J.A., Fabelo N., Fernandez C.E., Diaz M. - Membrane estrogen receptor as part of signalosomes in neurons. The concept of “lipid raft aging”.

• Tetel M.J. (USA) - Nuclear receptor coactivators in steroid action in brain

• Petersen S. (USA), Intlekofer K.A., Moura-Conlon P.J., Lopez J.A., del Pino J. - Non- classical progesterone signaling molecules in the nervous system

• Levine J. (USA) - Consequences of non-classical ER alpha signaling in brain

• Schütz G. (Germany), Berger S. - Dissection of glucocorticoid action in the brain using inducible gene inactivation

• Kajta M. (Poland), Rzemieniec J., Litwa E., Wnuk A., Lason W., Nalepa I., Zelek-Molik A., Wojtowicz A. - Impact of G-Protein-coupled receptor 30 on the effects of daidzein and dichlorodiphenyltrichloroethane in mouse embryonic neuronal cells