Plant Cell and Tissue Culture

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions in a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant (totipotency). Single cells, plant cells without cell walls (protoplasts), pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones. In DBT@MIET, we offer several modules of training as well as dissertation/project in Plant Tissue Culture. The duration of the programs offered for training ranges from 7 to 30 days and for project ranges from 3-6 months. Modules are selected by the students on their choice.

TRAINING

Module 1 (Code: PTC E-1): Basic Laboratory practices in Plant Tissue Culture

Duration- 7 Days ; Fee- Rs. 2000/-

Glassware Washing & Sterilization Techniques
Preparation of Culture Media
Sterilization of Explants
Culture of Explants In Culture Media
Callus Induction
Micro Propagation
Shoot Tip Culture

Module 2 (Code: PTC E-2): Skill development program in Plant Tissue Culture Technology

Duration- 15 Days ; Fee- Rs. 3500/-

Glassware Washing & Sterilization Techniques
Preparation of Culture Media
Sterilization of Explants
Culture of explants in Culture media
Callus Induction
Micro propagation
Shoot tip culture
Meristem Culture
Leaf culture
Embryo / Endosperm Culture
Somatic Embryogenesis

Module 3 (Code: PTC E-3): Job Oriented Advance Level Training Program in Plant Tissue Culture Technology

Duration- 30 Days ; Fee- Rs. 6000/-

Glassware Washing & Sterilization Techniques
pH Measurement & Preparation of Buffers
Preparation of Culture Media
Sterilization of Explants
Culture of Explants in Culture Media
Callus Induction
Shoot Tip Culture
Micro Propagation
Meristem Culture
Leaf culture
Nodule culture
Embryo / Endosperm Culture
Somatic Embryogenesis
Encapsulation of Callus
Cell Suspension Culture
Preparation of artificial seed

PROJECT/ DISSERTATION

We offer following Project/dissertation work depending upon the duration of the project.

Mini Project

Duration: 40 – 45 Days; Fee: Rs. 6,000 – 8000

Academic project

Duration: 2 – 6 month; Fee: Rs. 5,000 p.m

Research project

Duration: 2 – 6 month; Fee: Rs. 6,000 p.m.

A varied range of project/dissertation topics are offered in this division which features major techniques of Plant Tissue Culture viz:

Artificial Seed Production
Micropropogation of ovules, anther etc.
Callus Induction
Somatic Embryogenesis
Encapsulation

Contact @

+91-9761143975

arvind.kumar@miet.ac.in

Dr. Arvind Kumar

Dr. Arvind Kumar is working as Assistant Professor in the Biotechnology Department of MIET. He has been with MIET for more than 10 years and has a vast research experience in the area of plant science with an expertise in plant tissue culture research. He holds a Doctorate in the field of Plant Science and worked in reputed research institutions in India like Institute of Nuclear Medicine and Allied Sciences and Forest Research Institute. He has teaching experience of over 11 years in biotechnology and allied. He has guided many graduate and post graduate students in their research work and organized several national level symposia, workshops refresher courses and training programs. He is also the part of several academic bodies in MIET and also mentoring a young women scientist under DBT WOS-A scheme in the field of Nano Biotechnology at MIET. He has many research publications of national and international repute to his credit. He is life member of various academic bodies and editorial board member of reputed journals.

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