Publications

Articles published in 2024

Biotechnology | Food Science | Probiotics

Bioactive exopolysaccharide from Limosilactobacillus fermentum LAB-1: antioxidant, anti-inflammatory, antibacterial and antibiofilm properties

Nowshin Tarannum, Ferdausi Ali, Md Sajib Khan, Ohoud S Alhumaidan, ANMS Zawad, TJ Hossain*

Bioactive Carbohydrates and Dietary Fibre, 2024

| Author-note | We presented the multifaceted beneficial roles of our isolate, LAB-1 EPS. Previous genomic analysis had already indicated LAB-1's capacity for EPS production, and this study offers further evidence for this. The extracted EPS sample exhibited a remarkable array of properties, including potent antioxidant activity, broad-spectrum antimicrobial effects against both Gram-negative and Gram-positive bacterial pathogens, inhibition of biofilm formation in critical pathogens such as S. aureus and E. coli, anti-inflammatory activities, and emulsification properties. These findings significantly contribute to our understanding of EPS properties and present promising opportunities for practical applications. The versatility of EPS as a sustainable and eco-friendly alternative aligns perfectly with the growing demand for innovative products and processes.

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Prodigiosin | Antiviral | In-Silico

Prodigiosin Demonstrates Promising Antiviral Activity Against Dengue Virus and Zika Virus in In-silico Study

Tanjilur Rahman, Mohammed Sajjad Hossain Bappi, Tanim Jabid Hossain*

Analytical Science Advances, 2024

| Author-note | In this study, we present a comprehensive computational analysis of prodigiosin, a natural tripyrrole compound, as a potential antiviral agent against Dengue (DENV) and Zika (ZIKV) viruses. With the increasing global prevalence of DENV and ZIKV infections and the current lack of effective antiviral treatments, the search for novel therapeutics has become urgent.  Using advanced computational techniques, including molecular docking and molecular dynamics simulations, we analyzed the interactions between prodigiosin and key viral proteins, NS3 helicase and NS5 methyltransferase. Our findings reveal that prodigiosin exhibits promising antiviral potential, demonstrating strong binding affinities to these proteins, which may contribute to sustained viral inhibition. Notably, the compound formed multiple interactions, including hydrogen bonds, with active site residues in NS5 methyltransferase, enhancing its inhibitory effect. Additionally, prodigiosin’s favorable drug-likeness and ADMET profiles indicate its potential as a safe and effective antiviral candidate. 

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Plant-microbe interaction

Multifaceted plant growth promoting traits and abiotic stress resistance abilities exhibited by chrysanthemum rhizobacteria

Mohammad Nazmul Ahmed Chowdhury, Tanim Jabid Hossain*, Subrina Akter Zedny, Mohammed Sajjad Hossain Bappi, Minhajur Rahman, Md Hossen, Iqbal Hossain Nafiz, Raihanul Islam

Current Applied Science and Technology, 2024

| Author-note | Our research highlights the isolation and characterization of 34 bacterial strains from the root-associated soil of Chrysanthemum. We identified seven strains exhibiting multiple plant growth promoting traits, such as auxin production, phosphate solubilization, nitrogen fixation, and ammonia production. These strains belong to genera Pantoea, Staphylococcus, Acinetobacter, Bacillus, and Serratia. The selected strains were also tested for their ability to withstand various abiotic stresses, demonstrating resilience to osmotic and salt stress as well as temperature variations, which underscores their potential applicability under diverse environmental conditions.  Our findings suggest that these rhizobacteria could play a significant role in promoting plant growth and sustainability in agroecosystems. The strains' abilities to produce hydrolytic enzymes further enhance their potential utility as biofertilizers. This work provides valuable insights into the development of eco-friendly strategies for improving crop productivity and sustainability, which is crucial in the context of global agricultural practices.. 

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Probiotics | Lactic Acid Bacteria

Assessment of the In-Vitro Probiotic Efficacy and Safety of Pediococcus pentosaceus L1 and Streptococcus thermophilus L3 Isolated from Laban, a Popular Fermented Milk Product

Shanta Paul, Tanim Jabid Hossain*, Ferdausi Ali, Md Elias Hossain, Tasneem Chowdhury, Ibrahim Khalil Faisal, Jannatul Ferdouse*

Archives of Microbiology, 2024

| Author-note | In this study, we have assessed the probiotic potential and safety aspects of lactic acid bacteria (LAB) isolated from Laban, a traditionally consumed dairy beverage. The LAB strains exhibited antagonistic effects against bacterial and fungal pathogens, antioxidant capacity, and tolerance to low pH, bile salts, and phenols. Additionally, these strains have demonstrated adhesion ability to ethyl acetate, chloroform, and xylene, along with autoaggregation and coaggregation properties. Moreover, the isolates displayed promising technological attributes, such as milk coagulation and exopolysaccharide production, suggesting their potential to enhance the quality of dairy products.  Our research underscores the potential of LAB from Laban in imparting health benefits to consumers. The natural presence of these beneficial microbes in fermented dairy products, such as Laban, offers an added advantage for individuals aiming to enhance their health through diet. 

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Antimicrobial assay

Methods for screening and evaluation of antimicrobial activity: a review of protocols, advantages and limitations

Tanim Jabid Hossain*

European Journal of Microbiology and Immunology, 2024

| Author-note | The global concern surrounding antimicrobial resistance remains pressing, necessitating the discovery of novel and effective antimicrobial agents. This review delves into a range of established and emerging techniques, including disk diffusion assay, well diffusion, spot assay, cross-streaking method, poisoned food method, agar dilution, broth macrodilution and microdilution, resazurin assay, co-culture technique, time-kill kinetics, flow cytometry, TLC bioautography, bioluminescence assay and impedance measurement. Each method's principle, advantages, limitations, and significance in antimicrobial screening and evaluation have been discussed.   The review aims to provide researchers, professionals, and practitioners in medicine, infectious disease, clinical microbiology, biotechnology, and pharmaceutical fields with an understanding of these antimicrobial evaluation techniques. By elucidating the strengths and limitations of each method, this paper seeks to empower researchers in making informed decisions on selecting appropriate assays for the efficient identification of potential antimicrobial agents.

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Fermented beverages | Probiotics | Lactic Acid Bacteria

Fermented and dairy beverages of Bangladesh: a rich source of probiotic lactic acid bacteria

Tanim Jabid Hossain*, Md. Sajib Khan, Jannatul Ferdouse

Food Science and Applied Biotechnology, 2024

| Author-note | We explored the rich landscape of traditional fermented and dairy beverages in Bangladesh, emphasizing their role as valuable reservoirs of probiotic lactic acid bacteria. Highlighting their cultural significance, the paper uncovers the microbial diversity within these beverages, showcasing their antimicrobial properties, antioxidant effects, cholesterol-lowering abilities, and exopolysaccharide production. By connecting the traditional practices with modern genomic insights, this work underlines the global relevance of probiotic-rich beverages in promoting health and nutrition. 

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Biosurfactant | Oil-emulsification

Isolation of Biosurfactant Producing Bacteria from Oil-Spilled Soil and Characterization of Their Secreted Biosurfactants in Pathogen-Inhibition and Oil-Emulsification

Mohammad Zobaer, Ferdausi Ali, Md Nural Anwar*, Mohammed Sajjad Hossain Bappi, Takia Binte Bakar, Tanim Jabid Hossain, Md. Sajib Khan, ANM Shahriar Zawad

International Journal of Pharmaceutical Sciences and Research, 2024

| Author-note | In this research, we’ve isolated and selected two promising bacterial strains based on their biosurfactant production, and performed an intensive characterization of the strains, and their secreted surfactants. The partially purified biosurfactants had relatively high activity forming stable emulsions with different hydrocarbons and also showed good antimicrobial activity. Moreover, the biosurfactants exhibited high levels of pH, salinity and thermal stability, and ability to degrade diesel oil which indicate their prospect for application in bioremediation and oil recovery under extreme conditions.

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