2017 Syllabus

Lecture Schedule

1. 1. Introduction to Plant tissue culture-conditions to multiply plants in vitro-principles

   2. Sterilization techniques for plant tissue culture

   3. Nutritional requirements for plant tissue culture-Inorganic nutrients, carbon source, Vitamins, gelling agents

   4. Nutritional requirements for plant tissue culture-Undefined supplements, Plant growth regulators, pH of the medium

   5. Micropropagation-methods-steps, advantages, problems

   6. Low cost alternatives in micropropagation.

   7. Large scale micropropagation of ornamentals- chrysanthemum, carnation ,orchids, gerbera and rose

   8. Large scale micropropagation of leaf foliages- Philodendron, dieffenbachia

   9. Large scale micropropagation of medicinal plants- Coleus, Ocimum, Phyllanthus

   10. Large scale micropropagation of plantation crops-rubber, pepper, cardamom, date palm, arecanut

   11. Large scale micropropagation of potato, tapioca, sugarcane and banana

   12. Large scale micropropagation in woody perennials-Neem, Teak, Bamboo, Paulownia, Eucalyptus

   13. Large scale micropropagation of rare and endangered medicinal plants - any five plants

   14. Production of Virus free plants – shoot / meristem culture

   15. Virus elimination methods- Thermotherapy, cryotherapy and chemotherapy.

   16. Virus Indexing – Biological methods and physical methods

   17. Mid-semester examination

   18. Virus indexing by DNA based methods–PCR, nucleic acid hybridization test and ELISA test.

   19. Virus indexing by protein based methods–ELISA, Dotblot immunoassay.

   20. Case studies in vegetatively propagated crops for meristem tip culture-banana, tapioca and potato

   21. Rooting- in vitro and ex vitro rooting and hardening of TC plants.

   22. Physiological changes during hardening of TC plants

   23. Packaging and transport of tissue cultured plants -domestic and for export.

   24. National certification system for TC plants (NCS-TCP).

   25. Guidelines for Accreditation of Tissue Culture Production Facility

   26. Guidelines for Accreditation of Test laboratory for virus diagnosis and genetic fidelity testing of tissue culture raised plants

   27. Plant secondary metabolites and its classification

   28. Application of secondary metabolites

   29. Extraction and quantification methods for secondary metabolites.

   30. Plant Cell cultures for secondary metabolite production –steps

   31. Effect of precursor and elicitors in secondary metabolite production

   32. Hairy root culture and its applications

   33. Biotransformation using plant cell cultures

   34. Large scale production through bioreactors

Practical schedule

1. Plant tissue culture laboratory organization, equipments and chemicals

2. Preparation of stock solutions for different media

3. Micropropagation of sugarcane

4. Micropropagation of Banana

5. Microprogation of rose

6. Micropropagation of chrysanthemum

7. Micropropagation of forest tree species – Eucalyptus, Bamboo

8. Callus induction from medicinal plants – Phyllanthus, Solanum, Neem

9. Extraction of secondary metabolites and phytochemical assay

10. A demonstration on HPLC and GC-MS

11. Testing anti-microbial activity

12. NCSTCP-Procedures and Quality analysis by Genetic Fidelity test

13. Virus indexing in TC plants

14. A demonstration on hardening and field transfer of TC plants

15. Visit to commercial tissue culture laboratory

16. Project report preparation

17. Final Practical examination

To download the complete manual (ABT301), click here

Assignment topics (Click here)

List of Reference E - Books / Relevant literature

1. Plant Biotechnology and Genetics (Click here)

2. Plant Tissue Culture 1 (Click here)

3. Plant Tissue Culture 2 (Click here)

4. NCS - TCP Guidelines (Click here)

5. Cloning (Click here)