2017 Syllabus (PDF)
Lecture Schedule
Introduction and historical development of molecular biology (Click here)
Nucleic acids-composition and primary structure of nucleic acids (Click here)
Secondary structure-base pairing and base stacking, Tertiary structure (Click here)
Genome organization. Structure of prokaryotic and eukaryotic nuclear genomes-DNA packaging (Click here)
Structure of Organellar genome (Click here)
Repetitive and non-repetitive DNA-classification. Sequence architecture in repetitive DNA (Click here)
Gene-definition, Concept of Gene-classical and modern concepts (Click here)
Overview of central dogma of life (Click here)
Classical experiments in DNA replication (Click here)
Models and mechanisms of DNA replication (Click here)
DNA replication in bacteria, phages and eukaryotes (Click here)
Polymerase Chain Reaction-Principles and Applications (Click here)
Transcription-Prokaryotic RNA polymerase-sigma factors, promoters and consensus (Click here)
Transcription in prokaryotes-recognition- elongation and termination (Click here)
Transcription in eukaryotes-promoters, transcription factors, proximal elements and long range regulatory elements (Click here)
Mechanism of transcription in eukaryotes- initiation, elongation and termination (Click here)
Post transcriptional modification- mRNA processing – capping, cleavage and polyadenylation (Click here)
Mid Semester Examination
Splicing of mRNA – Group of introns-spliceosomes and alternative splicing (Click here)
Post transcriptional processing of rRNA and tRNA (Click here)
RNA editing-insertion, deletion and base substitution (Click here)
Genetic code-codons-Wobble hypothesis (Click here)
Ribosome structure-function and characteristics; Pooling of charged tRNAs (Click here)
Translation in prokaryotes-30S and 70S initiation complex formation (Click here)
Translation in eukaryotes-40S and 80S initiation complex formation (Click here)
Elongation-translocation -transpeptidation and termination of translation (Click here)
Proteins- primary, secondary and tertiary structures. Classification of proteins (Click here)
Regulation of gene expression and check points - overview (Click here)
Operon concept-lac operon as negative/positive gene regulation (Click here)
Gene regulation – Trp operon- repressible operon concept (Click here)
Regulation of gene expression in eukaryotes- DNA modification and other factors (Click here)
Tools and techniques in molecular biology-Overview. (Click here)
DNA sequencing-classical and automated DNA sequencing methods (Click here)
Molecular markers- PCR and hybridization based molecular markers (Click here)
Batch: 2024 Batch - B. Tech. (Biotech) Course start date: April 2025 / Mid-Semester date: / Final Practical Examination date: / Final Theory Date