2022 Syllabus (Click here)
Lecture Schedule
1. History of plant tissue culture (Click here)
2. Principles and concepts of Plant Tissue culture
3. Nutritional requirements for Tissue culture- Inorganic and organic additives
4. Plant growth regulators used in plant tissue Culture-Classes, examples and effects
5. Morphogenesis; Direct and indirect organogenesis; Direct and indirect somatic embryogenesis.
6. Applications of different plant tissue culture techniques
7. Callus culture and establishment of suspensions
8. Artificial seeds/Synthetic seeds -Types, Encapsulation Methods-Applications
9. Micro propagation methods-Stages-Applications
10. Micropropagation of field crops-Banana, sugarcane, Ginger and Potato
11. Micropropagation of ornamental crops- Gerbera, orchid, carnation, lilium
12. Micropropagation of tree crops- Neem, teak, Eucalyptus. Inducing juvenility in explants sources/ coppicing
13. Micropropagation of few medicinal plants (gymnema, garlic,gloriosa and aloe vera)
14. Low cost alternatives in plant tissue culture
15. Quality management of TC plants- Genetic Fidelity testing and Virus indexing methods –PCR, ELISA
16. National certification System for Tissue Culture Raised plants(NCS-TCP)-An overview
17. Mid semester examination
18. Virus elimination by meristem and meristem tip culture – Methods –Thermotherapy, chemotherapy and micro Grafting-Applications
19. Androgenesis–Anther and microspore culture-production of androgenic haploids-pathways, diploidization -examples –Rice, wheat and brassica and applications
20. Gynogenesis– Pollinated Ovule, ovary culture production of gynogenic haploids –Examples Onion, Cucumber and Sugar beet-applications
21. Wide hybridization - embryo culture and embryo rescue techniques, endosperm culture.
22. Protoplast culture - isolation and purification; Protoplast culture
23. Protoplast fusion; Somatic hybridization - Production of Somatic hybrids and Cybrids
24. Somaclonal and gametoclonal variations – causes and applications
25. In vitro mutation –procedures for screening mutants
26. Somaclonal variation and in vitro screening for development of disease resistance
27. Somaclonal variation and in vitro screening for development of biotic stress tolerance-one example each for salt, drought, herbicide and heavy metal
28. Cell culture for production of secondary metabolites-steps
29. Methods to enhance secondary metabolite production in cell cultures
30. Bioreactors used for plant cell cultures
31. In vitro germplasm storage and cryopreservation
32. Commercial Tissue Culture: Case studies and success stories
33. Market assessment and demand for plant tissue culture
34. Project planning and preparation, economics, government policies
Practical Schedule
Laboratory organization for Plant Tissue culture
Preparation of stocks - macronutrients, micronutrients, vitamins and hormones, filter sterilization of hormones and antibiotics.
Preparation of Murashige and Skoog medium.
Micro-propagation of plants by nodal culture
Micro-propagation of plants by shoot tip culture
Embryo culture to hasten germination
Embryo rescue to overcome incompatibility
Anther culture for haploid production591
Callus induction in tobacco leaf discs and establishment of suspensions
Sub culturing of callus, shoots, regeneration of shoots and, root induction
Acclimatization of tissue culture plants and establishment in greenhouse
Meristem tip culture
Virus indexing in tissue culture plants using PCR technique
Virus indexing in tissue culture plants using ELISA technique
Genetic fidelity testing
Visit to a commercial plant tissue culture lab
Final practical examination
Batch: 2020 M. Sc. (Biotechnology) / Course start date: 27-07-2022 / Final Practical Examination date: 21-11-2022
Batch: 2021 M. Sc. (Biotechnology) / Course start date: 06-06-2023 / Final Practical Examination date: 04-10-2023
Batch: 2022 M. Sc. (Biotechnology) / Course start date: 26-09-2023 / Final Practical Examination date: