ABT301 - Plant Biotechnology - 2+1
Lecture Schedule
Unit I: Basics of Plant Tissue Cutlure
Lecture - 01: Introduction (PPT / Notes)
Lecture - 02: Plant tissue culture: Concepts, history and scope (PPT / Notes) (Updated PPT)
Lecture - 03: Media and Culture Conditions and Sterilization techniques (PPT / Notes)
Lecture - 04: Regeneration methods - morphogenesis, organogenesis and embryogenesis (PPT / Notes)
Lecture - 05: Culture types - callus culture and cell suspension culture; shoot tip and meristem tip culture (PPT / Notes)
Lecture - 06: Anther and pollen culture; ovule and embryo culture (PPT / Notes)
Unit II: Applied Plant Tissue Culture
Lecture - 07: Micropropagation - banana and ornamental plants (PPT / Notes)
Lecture - 08: National certification and Quality management of TC plants (PPT / Notes)
Lecture - 09: Meristem tip culture (virus free plants) and anther culture (doubled haploids) (PPT / Notes)
Lecture - 10: Protoplast isolation and fusion- somaclonal variation-synthetic seeds (PPT / Notes)
Lecture - 11: Secondary metabolite production, invitro germplasm conservation (PPT / Notes)
Unit III: Basic Molecular Biology
Lecture - 12: Genome organization- prokaryotes vs eukaryotes (PPT / Notes)
Lecture - 13: Central dogma of life - Structure of nucleic acids (PPT / Notes)
Lecture - 14: DNA replication (PPT / Notes)
Lecture - 15: Structure of a gene (PPT / Notes)
Lecture - 16: Aminoacids and their classification and genetic codes (PPT / Notes)
Lecture - 17: Transcription, Translation and Protein Synthesis (PPT / Notes)
Lecture - 18: Regulation of gene expression, Operon concept (PPT / Notes)
Mid semester Examination
Lecture - 19: Blotting techniques and Polymerase chain reaction (PPT / Notes)
Lecture - 20: DNA sequencing methods (PPT / Notes)
Unit IV: Recombinant DNA Technology and Genetic Transformation
Lecture - 21: DNA manipulation enzymes: Polymerases, restriction endonucleases and ligases (PPT / Notes)
Lecture - 22: Different types of vectors: plasmids, phagemids, cosmids, BAC (PPT / Notes)
Lecture - 23: Construction of recombinant DNA molecules- Bacterial transformation (PPT / Notes)
Lecture - 24: Direct and indirect gene transfer methods in plants: microinjection, electroporation, particle bombardment, Agrobacterium mediated method (PPT / Notes)
Lecture - 25: Tissue specific promoters; Reporter genes: Selectable and Scorable markers, , Molecular analysis of transgenic plants (PPT / Notes)
Lecture - 26: Transgenic plants: herbicide, pest and disease resistant, abiotic stress resistant (PPT / Notes)
Lecture - 27: Transgenic plants: nutritional enhancement and traits for improved quality (PPT / Notes)
Lecture - 28: Detection of GMOs – regulations and biosafety (PPT / Notes)
Unit V: Molecular Marker Technology and Molecular Breeding
Lecture - 29: DNA markers - hybridization based markers (RFLP) - PCR based markers: RAPD, SSR, AFLP, and SNPs (PPT / Notes)
Lecture - 30: DNA fingerprinting of crop varieties (PPT / Notes)
Lecture - 31: Development of mapping populations (PPT / Notes)
Lecture - 32: Linkage and QTL analysis (PPT / Notes)
Lecture - 33: Principles, methods and applications of Marker Assisted Selection in crop improvement (PPT / Notes)
Lecture - 34: Applications of Plant Genomics and genome databases (PPT / Notes)
Practical schedule
Experiment No. 01: Biotech Laboratory organization, safety regulations (PDF)
Experiment No. 02: Basics of reagents and solution preparation (PDF)
Experiment No. 03: Plant tissue culture media preparation (PDF)
Experiment No. 04: Shoot tip culture of Rose (PDF)
Experiment No. 05: Meristem culture of Tapioca (PDF)
Experiment No. 06: Micro-propagation of banana (PDF)
Experiment No. 07: Callus culture (PDF)
Experiment No. 08: Isolation of bacterial plasmid DNA (PDF)
Experiment No. 09: Restriction Digestion and Ligation (PDF)
Experiment No. 10: Competent cell preparation and Bacterial transformation (PDF)
Experiment No. 11: Confirmation of transformation through colony screening (PDF)
Experiment No. 12: Genomic DNA extraction from plants (PDF)
Experiment No. 13: Quantification of DNA and quality check through Agarose gel electrophoresis (PDF)
Experiment No. 14: DNA fingerprinting using PCR (PDF)
Experiment No. 15: NTSys - analysis of diversity in crop plants (PDF)
For sample data for analysis, download here
Experiment No. 16: Visit to tissue culture units / biotech lab in seed industry / Bt cotton field – Lateral Flow Strip Assay (PDF)
Practical examination
To download the complete manual, click here
Assignment topics (Click here)
2020 - 2021: Batch - 2018 B. S. (ABM) / Course start date: 16-09-2020
2021 - 2022 : Batch - 2018 B. S. (ABM) / Course start date: 21-12-2021
2022 - 2023 : Batch - 2019 B. Sc. (Agri) / Course start date: 29-06-2022
2022 - 2023 : Batch - 2019 B. S. (ABM) / Course start date: 20-03-2023
2023 - 2024: Batch - 2020 B. Sc. (Agri.) / Course start date: 14-09-2023