2017 Syllabus (PDF)

Lecture Schedule

Unit I: Basics of Plant Tissue Culture

1. Introduction-History, scope and concepts of Tissue culture (Click here)

2. Sterilization techniques in plant tissue culture-methods, principles and uses (Click here)

3. Nutritional requirements and factors affecting plant tissue culture-explant, medium and other environmental factors (Click here)

Unit II: Pathways of Plant Regeneration

4. Pathways of plant regeneration-Morphogenesis, organogenesis-direct and indirect, method of plant regeneration (Click here)

5. Somatic embryogenesis-stages, induction, maturation and plantlet conversion-Synthetic seeds (Click here)

6. Callus culture-suspension culture-applications (Click here)

Unit III: Plant Tissue Culture Techniques for Propagation

7. Meristem culture and virus elimination-chemotherapy, thermotherapy, cryotherapy and applications (Click here)

8. Virus indexing methods-sap transmission, ELISA, nucleic acid hybridization, dot blot, PCR (Click here)

9. Mid Semester Examination

10. Micropropagation-stages and applications-Rose, Banana (Click here)

Unit IV: Organ Culture

11. Embryo culture-embryo rescue-technique and applications (Click here)

12. In vitro fertilization methods-Ovule , ovary and endosperm culture-applications (Click here)

13. Anther and microspore culture-pathways of haploid plant regeneration-doubling of haploids-applications (Click here)

Unit V: Plant Tissue Culture Techniques - Other Applications

14. Protoplast isolation-purification-fusion and culture methods-applications of protoplast culture (Click here)

15. Somaclonal variation-causes, types and applications (Click here)

16. In vitro germplasm conservation-slow growth and normal growth – applications (Click here)

17. Secondary metabolites through plant cell cultures-advantages-up scaling procedures-Bioreactors (Click here)

Practical Schedule

1. Basics in designing a plant tissue culture laboratory

2. Sterilization methods for plant tissue culture and surface sterilization of explants

3. Stock solution preparation

4. Medium preparation

5. Inoculation of leaves and seeds explants for callus induction

6. Inoculation of stem and young floral buds explants for callus induction

7. Meristem tip culture

8. Rose Micropropagation

9. Banana Micropropagation

10. Embryo culture

11. Sub culturing of Callus and shoots

12. Anther culture in rice

13. Establishment of suspensions and viability assay

14. Rooting and hardening of TC plants

15. In vitro selection for salt stress

16. In vitro selection for drought stress

17. Final Practical Examination

Batch: 2019 B. Tech. (Biotechnology) / Course start date: / Mid-Semester date: / Final Practical Examination date: 02-08-2022 / Final Theory Date