BTB202 - Plant Tissue Culture - (1+1)
2017 Syllabus (PDF)
Lecture Schedule
Unit I: Basics of Plant Tissue Culture
Introduction-History, scope and concepts of Tissue culture (Click here)
Sterilization techniques in plant tissue culture-methods, principles and uses (Click here)
Nutritional requirements and factors affecting plant tissue culture-explant, medium and other environmental factors (Click here)
Unit II: Pathways of Plant Regeneration
Pathways of plant regeneration-Morphogenesis, organogenesis-direct and indirect, method of plant regeneration (Click here)
Somatic embryogenesis-stages, induction, maturation and plantlet conversion-Synthetic seeds (Click here)
Callus culture-suspension culture-applications (Click here)
Unit III: Plant Tissue Culture Techniques for Propagation
Meristem culture and virus elimination-chemotherapy, thermotherapy, cryotherapy and applications (Click here)
Virus indexing methods-sap transmission, ELISA, nucleic acid hybridization, dot blot, PCR (Click here)
Mid Semester Examination
Micropropagation-stages and applications-Rose, Banana (Click here)
Unit IV: Organ Culture
Embryo culture-embryo rescue-technique and applications (Click here)
In vitro fertilization methods-Ovule , ovary and endosperm culture-applications (Click here)
Anther and microspore culture-pathways of haploid plant regeneration-doubling of haploids-applications (Click here)
Unit V: Plant Tissue Culture Techniques - Other Applications
Protoplast isolation-purification-fusion and culture methods-applications of protoplast culture (Click here)
Somaclonal variation-causes, types and applications (Click here)
In vitro germplasm conservation-slow growth and normal growth – applications (Click here)
Secondary metabolites through plant cell cultures-advantages-up scaling procedures-Bioreactors (Click here)
Practical Schedule
Basics in designing a plant tissue culture laboratory
Sterilization methods for plant tissue culture and surface sterilization of explants
Stock solution preparation
Medium preparation
Inoculation of leaves and seeds explants for callus induction
Inoculation of stem and young floral buds explants for callus induction
Meristem tip culture
Rose Micropropagation
Banana Micropropagation
Embryo culture
Sub culturing of Callus and shoots
Anther culture in rice
Establishment of suspensions and viability assay
Rooting and hardening of TC plants
In vitro selection for salt stress
In vitro selection for drought stress
Final Practical Examination
Batch: 2019 B. Tech. (Biotechnology) / Course start date: / Mid-Semester date: / Final Practical Examination date: 02-08-2022 / Final Theory Date