Video Protocols

VDS YouTube channel (most videos posted here)

Wet Lab Video Protocols

Lab Walk Through Video (5 min)

How to Program a PCR Machine (10 min Panopto)

VDS FRI Glass Washing (5 min)

VDS FRI Spectrophotometer (3 min)

VDS FRI Nanodrop (Proteins) (5 min)

VDS_NiNTA_columnsReusing (3 min)

Protein Expression - Syringe Sterile Filter (from 2011) (5:30 min)

A quick video walk through of DSF Lysozyme calculations - Dr. B ~11 min (2021)

Autoclave  (video TBD)

- written Autoclave Protocol

Beckman Centrifuge Protocol Overview VDS (w/ Speed charts) - Dr.B ~10min 

Centrifuge 101 VDS (2024) - (18:17 min)

SpreadingBacteriaPlates Cotton Swab (1:39 min)

SpreadingBacteriaPlates Cotton Swab v2  (2:20 min)

Antibiotic Discs application   (1:38 min)

Plasmid Archive & Plasmid Stocks - VDS (3:30 min)

pHMeterCalibration VDS (4:35 min)

VDS pH Meter Calibration (Orion) - new pH meter 2/28/25 - Antonia & Raina (~5 min)

Primer & GBlock Dilution VDS Dr. B (9 min)

Old Video Protocols folder on Google Drive  ( TBD - upload these to VDS Staff YouTube account and link here as videos)

• these have been moved to Dr. B's UTBox and are ready to be uploaded to YouTube (1/30/24)

Video Protocols To Be Done:

Sonicator

Sterile technique & using burners (transformation) - see Spreading Bacterial Plates (not quite the same)

Making an agarose gel 

Liquid Nitrogen w/ Snap Freezing

Virtual Video Protocols

Downloading PyMOL to Windows PC v2 (5 min)

PyMol License File (1st time or renewing an expired license) - Dr. B 

VS1 DHFR Gold Via Hermes (18 min)

Set Up and Run GOLD for Mac (11 min)

Text Import Wizard for Excel and Bestranking List (7 min)

VS1 Part2 VDS (44 min)

Alignment in PyMol with Advanced PDB Search - VDS DrB (14 min)

VS1 Mac BCRF Walkthrough (14 min)

VS1 PC (Windows) Software Download Walkthrough (5 min)

VS1 PC (Windows) BRCF (15:25 min)

VS1 Mac Software Download Walkthrough (7 min)

VS1 Part1 VDS - Intro (20 min)

Docking Strategy ex. DHFR (13min) - Panopto

TBD: Docking Strategy v2 ex 6PGDH  ex DalaDala  ex allosteric