Mass Photometer

Although this is not actually a mass spectrometer (It is an optical technique ) it is housed in the Mass Spectrometry Facility WEL 4.218) as it serves a similar function.

The RefynTwoMP measures the mass of single molecules in solution, ​including both proteins and nucleic acids. It works by measuring Rayleigh scattering of molecules and correlating scattering contrast to mass using the contrast and mass of a known calibrant. It is capable of measuring complexes from 30 kDa to 5 MDa with a working protein concentration range of 100 pM to 100 nM with a resolution (FWHM) of 25 kDa at 66 kDa and 60 kDa at 660 kDa. It is especially useful to study native complexes in a wide range of buffers. This instrument is housed in the mass spectrometry facility in WEL 4.218. Training is available by email.