BD FACSMelody Digital Flow Cytometry sorter 3 laser system

This  compact yet powerful instrument, capable of detecting up to 10 parameters and 8 colors simultaneously, makes it possible to identify and isolate even the rarest cell population you are interested in. Samples can be sorted at single cell-basis with sample agitation and temperature control into two or four tubes (5mL round bottom tube to 1.0mL eppendorf), multi-well plates or microscope slides. Our Melody system is housed in a Class-II Type A2 bio-safety cabinet to ensure that samples remain sterile during sorting process. It is capable of accommodating most cell types with its 70, 85 and 100 micron nozzles.

Location: Room 451 (level 2+), Apotex Center, University of Manitoba Bannatyne Campus, 750 McDermot Ave, Winnipeg, MB.

Note: this instrument is inside the BSL2+ lab, so only trained users who are on the permit of that room is allowed to stay and work in the room.  Those dropping off the samples need to leave the room as soon as they confirm their sample and gating with Dr. Christine Zhang

3 Lasers:

• 1. 20mW 488nm direct diode blue laser,

  2. 20mW 405nm direct diode violet laser, and

  3. 50mW 561nm Solid State violet laser.

17 parameters:

• • 1-2 Six fluorescent detectors off 488nm,

  • 3-4. Three fluorescent detectors off 405nm,

  • 5-8. Six fluorescent parameters off 405nm,

  • 9.  Forward scatter, and

  • 10. Side scatter.

Acquisition/Analysis software: FACSChorus

Notes:

• • How to read longpass filter detection range

    • e.g., 502LP—wavelength Longer than 502nm will be allowed to Pass through

  • How to read bandpass filter detection range

    • e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm