BD FACSCanto-II Digital Flow Cytometry Analyzer 3 Laser System

This powerful instrument, capable of detecting up to 10 parameters and 8 colors simultaneously, is ideal for both clinical and research applications. Its newly designed loader carousel allows for automatic loading of up to forty 12x75mm tubes without operator intervention, reducing hands-on time and improving results reliability.

Location: Room 466, Apotex Center, University of Manitoba Bannatyne Campus, 750 McDermot Ave, Winnipeg, MB.

Sample Handling

• • Semi-automatic Single Tube Loading with 12 x 75 mm tubes

3 Lasers:

• 1. 20mW 488nm Solid State blue laser,

  2. 17mW 633nm HeNe red laser, and

  3. 30mW 405nm Solid State violet laser.

10 parameters:

• • 1-4. Four fluorescent detectors off 488nm,

  • 5-6. Two fluorescent detectors off 633nm,

  • 7-8. Two fluorescent parameters off 405nm,

  • 9. Forward scatter, and

  • 10. Side scatter.

Acquisition/Analysis software: FACSDiva

Note:

• • How to read longpass filter detection range

    • e.g., 502LP—wavelength Longer than 502nm will be allowed to Pass through

  • How to read bandpass filter detection range

    • e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm