BD FACSAriaIII Digital Flow Cytometry sorter 3 laser system

This powerful instrument, capable of detecting up to 17 parameters and 15 colors simultaneously, makes it possible to identify and isolate even the rarest cell population you are interested in. Samples can be sorted at single cell-basis with sample agitation and temperature control into two or four tubes (15mL centrifuge tube to 1.0mL microtubes), multi-well plates or microscope slides. It is equipped with Aerosol Management Option (AMO), a device that traps aerosolized particles. Our Aria-III system is housed in a Class-II Type A2 bio-safety cabinet to ensure that samples remain sterile during sorting process. It is capable of accommodating most cell types with its 70, 85 and 100 micron nozzles.

Location: Room 462, Apotex Center, University of Manitoba Bannatyne Campus, 750 McDermot Ave, Winnipeg, MB.

3 Lasers:

• 1. 20mW 488nm Solid State blue laser,

  2. 15mW 633nm HeNe red laser, and

  3. 30mW 405nm Solid State violet laser.

17 parameters:

• • 1-6. Six fluorescent detectors off 488nm,

  • 7-9. Three fluorescent detectors off 633nm,

  • 10-15. Six fluorescent parameters off 405nm,

  • 16. Forward scatter, and

  • 17. Side scatter.

Acquisition/Analysis software: FACSDiva

Notes:

• • How to read longpass filter detection range

    • e.g., 502LP—wavelength Longer than 502nm will be allowed to Pass through

  • How to read bandpass filter detection range

    • e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm