Biosafety

All flow cytometry instruments in the facility are housed at bio-safety level 2 (BSL2) labs. Therefore, only live samples with BSL no higher than level 2 are allowed to enter the facility. Samples from risk group BSL3 or higher must be pre-treated with paraformaldehyde before accessing the facility and the facility manager must be informed in advance. The user need to ensure that enough bleach is present in the waste tank of the instrument during acquisition when using the flow cytometry analyzers (FACSCanto and LSR-II).

The users must have their formal bio-safety training completed and lab permits available before using the facility. The facility manager reserves the rights to:

• • request for clarifications and BSL documents from the facility users when necessary, and/or

  • reject the entrance of certain samples from the users if bio-safety regulations are not adhered to.

The FACSAria-III is housed in a Class II type A2 bio-safety cabinet to ensure proper aerosol management of the sample during sorting.

Contact Dr. Christine Zhang for details if you have further questions or concerns.