Cytek Aurora Spectral Flow Cytometry Analyzer 5 Laser System

Cytek Aurora spectral flow cytometry analyzer is equipped with 5 lasers and  capable of detecting up to 64 fluorescent parameters, giving researchers more flexibility and room to easy design a large panel of markers of over 20 colors. Besides the standard forward and side scatter channels off from blue laser, it also comes with side scatter channel off from 100mw 405nm violet laser.  Other than the conventional individual 75mm round bottom tube loading, Aurora also comes with high throughput Automated Sample Loader, compatible for 96 well plates, 96 deep-well plates and 40 tube racks to streamline the acquisition work.

Location: Room 531, Basic Medical Science Building, University of Manitoba Bannatyne Campus, 7745 Bannatyne Ave, Winnipeg, MB.

Sample Handling

• • Semi-automatic Single Tube Loading with 12 x 75 mm or 1.2 mL tubes

  • High Throughput Automated Sample Loader unit for 96 well plates, 96 deep-well plates and 40 tube racks

5 Lasers:

• 1. 50mW 488nm Solid State blue laser,

  2. 80mW 640nm Solid State red laser

  3. 100mW 405nm Solid State violet laser.

  4. 50mW 561nm Solid State yellow-green laser

  5. 20mW 355nm Solid State UV laser

67 parameters:

• • 1-14: fourteen fluorescent detectors off blue laser

  • 15-22: eight fluorescent detectors off red laser

  • 23-38:  sixteen fluorescent detectors off violet laser

  • 38-48: ten fluorescent detectors off yellow-green laser

  • 49-64: sixteen fluorescent detectors off UV laser

  • 65: Blue Laser Forward scatter 

  • 66: Blue Laser Side scatter

  • 67: Violet Laser Side scatter

Acquisition/Analysis software: SpectroFlo

Note:

• • How to read bandpass filter detection range

    • e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm