BD LSRFortessa Digital Flow Cytometry Analyzer 5 laser system

This instrument is capable of detecting up to 18 fluorescent parameters, making it ideal for multicolor flow cytometry assays that involve a large panel of markers. It comes with High throughput Sampler Unit for sample acquisition in multi-well plate

Location: Room 531, Basic Medical Science Building, University of Manitoba Bannatyne Campus, 745 Bannatyne Ave, Winnipeg, MB.

Sample Handling

• • Semi-automatic Single Tube Loading with 12 x 75 mm or 1.2 mL tubes

  • High Throughput Sampler unit for multi-well plates (standard and high throughput modes)

3 Lasers:

• 1. 50mW 488nm Solid State blue laser,

  2. 40mW 640nm Solid State red laser

  3. 50mW 405nm Solid State violet laser.

  4. 50mW 561nm Solid State yellow-green laser

  5. 20mW 355nm Solid State UV laser

20 parameters:

• • 1-2: Two fluorescent detectors off blue laser

  • 3-5: Three fluorescent detectors off red laser

  • 6-11: Six fluorescent detectors off violet laser

  • 12-15: Four fluorescent detectors off yellow-green laser

  • 16-18: Three fluorescent detectors off UV laser

  • 19: Forward scatter 

  • 20: Side scatter

Acquisition/Analysis software: FACSDiva

Note:

• • How to read longpass filter detection range

    • e.g., 502LP—wavelenge Longer than 502nm will be allowed to Pass through

  • How to read bandpass filter detection range

    • e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm