Research methodology

Hypthesis

Research hypotheses are the specific testable predictions made about the independent and dependent variables in the study. Usually the literature review has given background material that justifies the particular hypotheses that are to be tested. Hypotheses are couched in terms of the particular independent and dependent variables that are going to be used in the study.

Tips for methodology

• Do not over-explain common scientific procedures. For example, you do not need to explain how PCR or Western Blotting work, just that you used the techniques.

• If you are using a novel technique, then you need to explain the steps involved. Use third person passive tense. For example, “RNA was extracted from the cells.” Compare this with, “We extracted RNA from the cells.”

• Be sure to mention from which companies you purchased any significant reagents for your experiments.

•  When in doubt about how to report your materials and methods, look to papers published in recognized journals that use similar methods and/or materials.

• Do not mention sources of typical lab ware (beakers, stripettes, pipet tips, cell culture flasks, etc)

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