Embedded Files
Make a copy of the SOP to take your own notes!
Make a copy of the SOP to take your own notes!
Quick Reference Steps
Quick Reference Steps
Sample Preparation
Sample Preparation
Secure the sample to a pin stub, if possible.
Ensure the sample is clean.
System Startup
System Startup
Log in to NEMO.
Vent the chamber.
Load the sample onto the stage. Do not overtighten set screws.
Load the detector.
Set the pressure and pump the chamber.
Move the sample surface to 10mm. Activate the chamberscope and move in Z with the mouse.
Check that the stage is close to (0,0) in (X,Y.)
Once the chamber is at vacuum, set the voltage and turn on the beam.
Alignment and Imaging
Alignment and Imaging
Zoom out to low magnification
Navigate to the sample then gradually zoom and focus.
Adjust brightness and enhance as needed (or contrast in high vacuum only.)
With the image in focus at sufficiently high magnification, link the stage and working distance.
Adjust initial conditions as needed (voltage, pressure, spot size.)
Align the aperture with Lens Align.
Align the stigmators with the stigmator knobs or crosshair.
Navigate the sample, focus, and save images.
You may need to adjust alignment or settings as you move, zoom in, or change the voltage or spot size.
Shutdown
Shutdown
Turn off the beam.
Return stage tilt to 0.
Vent the chamber.
Remove the sample from the stage.
Return the normal pin stub stage if another was used.
Remove the detector (if used) and return it to the dry box.
Pump the chamber.
Log out of NEMO.
Copy data in a timely manner.
Clean up after yourself.
Keyboard Shortcuts
Keyboard Shortcuts
- F5: toggle between full screen and quad view.
- F6: pause/unpause the selected window.
- F7: toggle between full screen and a reduced area view.
- F2: Photo (captures an image with a long dwell time over 1 frame.)
- F4: Snapshot (captures an image with short dwell time integrated over N frames.)
- [ctrl + >]: faster scan (shorter dwell time.)
- [ctrl + <]: slower scan (longer dwell time.)
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