During the presentation the topics that will be covered are the steps taken to make conclusions about the mutated amino acid.

Transform competent BL21 E. coli cells with plasmids containing mutated sequences of the ꞵ-Glucosidase gene BLR cells are a strain of E. coli that result in a particularly high level of protein production. Grow transformed cells and induce protein production using IPTG which triggers the transcription of lac operon. Extract protein using affinity chromatography this also serves as a method of purification. Perform a protein assay to determine concentration ad the yield of the protein to allow for analysis. Analyze protein purity by using a kinetic assay the purity of the enzyme will be based on the color change in the color change over time. Finally, form a conclusion about the overall purity and kinetic activity after the amino acid residues have been changed. 

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Transforming competent BL21 E. Coli cells with plasmids containing mutated sequences of the beta-glucosidase gene. These cells will be transformed and grown to induce protein production. The protein will extracted using affinity chromatography. Then a protein assay will be used to determine the concentration of the protein produced. Finally ending with analyzing the purity of the protein, which would allow us to analyze the kinetic activity using a colorimetric kinetic assay.

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I'd like to outline the dichotomy of Iranian and American foreign policies, and explore their differences. I'd then discuss how these differences come to fruition in cyberspace in the form of conflict. I'd finally bring up the implications of this strenuous relationship and offer possible resolutions.

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The number of Americans who identify themselves as separate from a religion doubled from 7 to 14 percent in the 1990s, and it has been on a steady decline ever since. Today, we are far beyond postmodernism; we are living in the results and the consequences that came from that era, as the world entirely is completely transformed in many aspects. From the creation of new world organizations for the prevention of past worldly historic mistakes, to the granting of more universal rights across developed states, the people were given more freedom to be, which meant that there was no longer a pressure necessary to follow a traditional religion nor the need for the comfort that was once so vital. Religion in America is what led to the foundation of the institutions and the type of government which exists here today but current numbers show that approximately 89% of the population does believe in God, despite the fact that numbers are evidently decreasing- furthermore, it is important to note that Americans identify as spiritual but with no specific religion. This decline of religion in the United States has shone evidently on younger generations globally who are losing their religion, which may be due to their upbringing by generations raised in a post-war world, the transformation of social politics and the role which religion still plays in it, the theory of secularization, and the appeal towards other social communities.

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Cultural traditions form the foundation of a society, but traditions also undergo shifts to adapt to broader societal, economic, and political changes through time. Chanoyu, as one of the most well-known Japanese traditions, is a prominent example of a tradition’s change over time. In response to westernization, Okakura Kakuzō published _The Book of Tea_ in 1906, which signified chanoyu’s transition from common practice to a symbol of Japanese culture. Okakura proposed that chanoyu is not only a product of Japanese society, but also the essential embodiment of Japanese culture, art, and philosophy, and therefore must be preserved (Okakura 9-10). Then and today, chanoyu’s practitioners balance the desire to honor chanoyu’s roots, while also adapting it to fit contemporary themes, like technologization and globalization. To adapt, I argue that, by drawing from Okakura’s philosophy, chanoyu’s meaning has changed to embody Japan’s cultural identity and a way to reconnect with traditional Japanese culture, also known as premodern Japan. Chanoyu’s practitioners seek to align chanoyu with the traditional Japanese values of harmony, respect, purity, and tranquility. They prioritize elements that align with these values, specifically emphasizing the authority of the iemoto system and its ideologies, while assigning little importance to chanoyu’s contemporary developments (Surak 91). By doing such, chanoyu can be championed as a force against urbanization and a way to reclaim what it means to be Japanese. Ultimately, this phenomenon speaks not only to chanoyu’s role in Japan, but also to the growing importance of traditions and traditional values in 21st-century Japan. 

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During the Mozambique war for independence, FRELIMO, the organizing party of the Mozambicans, was able to mobilize women into their cause and drastically change the tides of war. FRELIMO was fighting an uphill battle for independence with the occupying Portuguese. However, the party's leader declared that the integration of women was necessary for the state's survival. Through ideological and practical strategies, FRELIMO effectively recruited female agents en masse and empowered the 'forgotten 50%.'

The propaganda arm of FRELIMO recruited female agents, utilizing a variety of female-oriented propaganda that appealed to women. Women were portrayed as strong, capable, and active participants in the revolution through various mediums, such as radio programs, magazines, and pamphlets. In addition, FRELIMO expanded clinics and hospitals in rural areas, staffing them with women, not as nurses, but as doctors (Hansen 240-41). Aside from Frelimo introducing one of the very first gender quotas for commanding officers and politicians (Penicela 41). They also had women acting in combat roles, getting special military and political training at special centers (Penicela, 22).

On the homefront, women continued to be a priority; even during the revolution, FRELIMO set up women detachments of the party to oversee women's education in business skills and literacy to increase their independence, as well as supplied many rural women loans to start them (Hansen, 231). These initiatives went beyond addressing the gender inequality so prevalent in Mozambique at the time but also served as a necessary step towards liberating the country. 

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Parkinson’s Disease is a difficult neurodegenerative disorder that takes a heavy toll on not only motor-function but also many other aspects of a patient’s well being. The primary treatment method for this disorder is almost solely based on medication. This study aims to institute the efficacy of maintaining a healthy lifestyle by way of maintaining proper nutrition and regular exercise as an additional branch of existing treatment. This would incorporate a more natural form of treatment that would further ensure that patients are being provided with the most well rounded care possible that prioritizes their quality of life. Results were collected from an in depth analysis of a longitudinal study as well as three qualitative assessments. This analysis found that with respect to many different non-motor symptoms of Parkinson’s, taking actions that promote a healthy lifestyle has shown to decrease the progression of these symptoms and provide patients with a better overall quality of life in comparison to patients using medicinal treatment alone. This profound evidence highlights the immense value of Parkinson’s patients having access to fitness and nutrition specialists to help guide and regiment them into practicing a healthy lifestyle since the nature of this disorder makes this very difficult for them to do all on their own. The implication of these findings is that treatment for Parkinson’s Disease can be vastly improved by giving patients access to the fitness and nutritional resources they desperately need to maintain a healthy lifestyle. This study can help further research on forms of treatment for the non-motor symptoms of Parkinson’s disease ensuring that these are being given as much attention in treatment strategies as their motor counterparts.

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Chiari Malformation One is a very challenging condition, with over one in a thousand possessing this, and not discovering surgery option until later. Surgery is highly recommended for those with severe cases. This study aims to establish the efficiency and improvement patients feel when having underwent surgery for Chiari Malformation. To obtain these results, one book, fourteen scholarly reviews, and many clinical trials were analyzed. This anaylsis found that over three fourths of patients that received the surgical treament experienced postive changes. Many experienced an improved quality of life doing everyday tasks, minimized to no heaaches, etc. Research indicates that the younger surgery is completed the more favorable the outcome is. Studies show that many individuals do not discover they possess this condition until at a much older age which may cause results to vary. From a younger perspective cognition and routine physical performance is exceptional post-operation. Furthermore, studies found that neurological status is increased allowing neurons to fire and more cerebrospinal fluid to flow within the craniovertebral junction. CM1 surgery is very effective within younger teens and as age increases, surgery is still effective, just not at the equivalent of the younger groups. The implications of these findings is that CM1 must become a larger topic of discussion among physicians due to treatment being effective within pediatrics. This study can contribute to further research in improving how doctors assess this condition, and performing X-rays at younger ages in life in order to ultimately assist patients as early as possible.

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Consumers of fast food might have a hard time staying away from their favorite businesses due to the strategic psychology the businesses use to influence them. In order to find the causes of manipulation that fast food businesses use to increase their sales, five scholarly article reviews were analyzed. This analysis found that color plays a major role in fast food advertising as it induces hunger and evokes emotion. Not only this, but colors can affect the way people view certain foods and cause them to feel like they are healthier than they think. This would be known as cognitive dissonance because their attitudes and beliefs start to conflict. Research has also shown that positive emotions in a product can make it more attractive and create positive attitudes towards the product. These findings imply that colors are very important to fast-food advertising. Not only does it evoke emotions and hunger, but it attracts customers into buying more than they need. Some future research that could be performed is whether the purchase of fast food is related to more cognitive behaviors or motivational behaviors. This research can be applied to almost anything since there are colors around everything. People might benefit from learning how cognitive dissonance plays a role in the purchasing process because it could be able to help them in deciding whether they should purchase fast food or not. 

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Multilingualism is a common ability of many individuals that comes along with different experiences in life and even in the brain. Using scholarly psychological journals, this research finds that the age at which dementia and Alzheimer’s develops among monolinguals vs bilinguals is significantly different. However, the likelihood of developing these conditions is not significantly different. While it could delay the diagnoses, multilingualism won’t prevent these conditions altogether. Nevertheless, this aspect of brain health seems to be benefited by speaking multiple languages. When tested in critical thinking disposition, the difference between monolinguals and bilinguals is not significant during younger years of life. However, as they get older the difference is more noticeable and the advantages of bilingualism are revealed. Despite this, using language learning as a method to counteract age-related cognitive decline has proven to be ineffective, so prescribing language learning in old age would not be a proper treatment. In order for it to be effective, language learning would need to take place at a younger age so that fluency could more easily be achieved, in which case it could be used as a preventative measure rather than as a treatment. Language learning should be encouraged the most during the formative years of development and should be part of education from the very beginning rather than later on. This will ultimately allow the benefits of multilingualism to occur and in turn produce a future society that experiences a more successful cognitive aging. 

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For this project I will cover how different types of music impact mental illness and brain disorders on a biological level. In specific I will be looking at the change in brain activity when certain music is played. I will then look at the brain activity of people with mental illness and brain disorders to look at possible ways music may affect their brains. Furthermore, people's preference for music will also be taken into account when looking at how music can affect the brain. Lastly, I will discuss the different components to music therapy and the different effects they can have on an individual. 

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How much choice is there in our gender identity – how is it affected by our living conditions, family conditions/ideals, and our brain? This study aims to examine how gender identity is positively or negatively influenced by living conditions, family conditions/ideals, and the brain. To achieve this aim, I analyzed five scholarly journals containing gender identity, implications of gender-affirming hormone therapy (GAHT), and the dichotomous effect of parent’s acceptance/rejection. This analysis found that gender identity is a life-altering, mostly once-in-a-life-time choice, but it is heavily influenced by hormones in our brain. In addition, families do not influence their child’s gender identity, but they do affect their child’s acceptance/confidence in his/her gender identity, causing their child’s life course to change. Research states that transgender individuals tend to block their transition to puberty through gender-affirming hormone therapy (GAHT) and that has beneficial mental effects. Furthermore, research found that GAHT helps induce desired secondary sex characteristics in transgender individual and helps achieve their desired appearance, causing their mental health to improve. Additionally, transgender individuals who “come out” to their family have their family relationships and their mental health greatly impacted. The implications of these findings are that social environment and socioeconomic status greatly affect the lives of transgender individuals. Being homeless or pursuing higher education typically resulted in opposite treatment by the families (Schmitz & Tyler, 2018). We need to have better acceptance of other’s gender identity – regardless of social environment, socioeconomic status, or pursuit of higher education.

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The Korean Wave refers to the explosion in popularity of South Korean pop culture in the US, specifically through social media and entertainment. This study aims to explore the effect that this has had on the acculturation process for new Korean immigrants. The following results were obtained by analyzing three journal articles, a qualitative study, and a dissertation. Upon conducting this analysis, it was found that young Korean Americans feel marginalized in the US and Korean media serves as a space for them to feel comfortable with their cultural identity. Additionally, the consumption of Korean media cultivates global imagination within immigrants which motivates them to seek out new relationships within the Korean American community. The growth of Korean pop culture also gives immigrants more opportunities to introduce Korean culture to Americans in a diverse set of social environments. Conversely, continuous consumption of Korean media decreases the socioeconomic factors that help immigrants assimilate into American culture. As a result, a bicultural identity is formed which can lead to frustration with self-identity as well as difficulty fitting in with either culture. From these findings, it can be concluded that Korean immigrants should be encouraged to utilize the Korean Wave through social media as a tool to grow friendships and create more opportunities to connect with other Korean Americans. This will lead to an increased global imagination which others should take advantage of by providing them with local events and gatherings to connect with people going through similar experiences. 

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Parentification can be detrimental to a child’s development and there remain questions surrounding what becomes of a parentified child once they reach adulthood, especially once they raise children of their own. In this paper, severe emotional parentification was focused on due to it being found to have the most detrimental effects on youth. This study analyzes thirteen different research papers to determine the most likely outcome of an adult parentified child’s parenting techniques. The journals examine the impact of neglect, parentification, and attachment on the futures of children who experience parentification, along with the causes behind neglect. Through this analysis, it was found that parentification can be linked to attachment insecurity due to similar outcomes and causes, while also being able to be connected to histories of mental illness in the parentified child’s parents. Neglectful parenting styles are employed by those that have insecure attachments, were parentified, or were neglected. Also, less beneficial parenting techniques were used by those with symptoms that are commonly caused by severe emotional parentification. Extreme parenting techniques have been shown to transfer down generations, and according to the research conducted, parentification likely follows suit. Thus, parentified children are most likely to use an extreme parenting style with their future children, such as permissive, neglectful, or authoritarian. These findings imply that the sources of parentification need to be further uncovered for better treatment and therefore break the intergenerational cycle of trauma and neglectful parenting. Understanding what makes parentification harmful can help parents form healthier boundaries.

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The search to "cure" anxiety is a goal those in the psychological community have been aiming to achieve forever, and presently seems to be more crucial than ever in aiding our development of a healthy society. We have found that out of all mental ailments Anxiety is one of if not the easiest to fix. Nonetheless, this poses the question which form of cognitive-based based therapy is the most effective in lessening the symptoms of anxiety disorders, specifically TBCT and MCBT? My approach to this growing issue is to move away from the chemical approach and focus more on CBT’s. This unfortunately is easier said than done due to there being a major lack of people in the mental health field qualified to do CBT. Another variable we must consider is that people are very different and respond to therapy much differently than others. Genetics' play a big part in why others are more susceptible to anxiety than other for example a more active central nervous system causes one to be more anxious. Another example of a variable hindering how well my plan could work, is the stigma behind therapy. Many like those in the military view it as weak and won't give therapy the time of day. But, if the U.S. puts more money into cognitive based talk therapy, we will see a decline in the need for medication, and see similar, if not better, results if cognitive therapy is the main form of treatment for those with anxiety disorders. 

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I will be taking close look on how mental health affects one's economic well-being. I will be looking at not only how government plays a role in economic areas, but how individuals with poor mental health or anxiety are monetarily affected. 

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Psychopathy is a severe form of antisocial personality disorder which is believed to have its roots in biology and highlights a distinct lack of proper moral development. A major contribution to the increased severity of symptoms in at-risk children is the history of adverse childhood environments, specifically abuse from parents or caregivers. The purpose of this study is to examine various research articles and studies to discover how parenting plays a role in shaping the moral development of a child at-risk to psychopathy, and the intensity of the child’s symptoms. The findings indicate that psychopathy, which is presumed to originate from genetic variations, is actually heavily influenced by environmental factors as well, and is enhanced and dangerous when an at-risk child faces extreme parental abuse and neglect. Consequently, the witnessing of or the experiencing of adverse childhood maltreatment in at-risk children produces particularly poor moral development and can cause the child to be unable to function normally with society, even becoming a potential antisocial hazard. These findings imply the extreme importance of positive parenting for children at risk to psychopathy. It also implies that the genetic inheritance of psychopathy cannot be the sole cause of poor moral development in these children, because abusive forms of parenting impacts the developing child’s brain in significantly important ways that intensify pre-existing traits of psychopathy and immorality.

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I will survey 5 journal articles to correlate if having a lower first exam impacts students motivation or performance levels throughout the semester. The project will also cover how differing motives for taking the class can impact some ones overall motivation or performance after the first exam. Having a lower test score on the first exam has been shown to have a significant impact on college students' performance and motivation when they were extrinsically motivated. However, college students who were intrinsically motivated had no apparent effect on motivation or performance in the class. Using these findings I hope to find out why certain motives for taking a class effects some ones ability to perform. 

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Mental health issues in the homeless population is a major, and growing concern in America. This study aims to find what the effects of mental health have on each different category of the homeless population, and how it can be specialized to meet the unique psychological needs of each category. To acquire the specific results needed for this experiment, 5 scholarly sources were thoroughly examined to find the needed results. Research from several articles found that when Sexual and Gender minorities (SGM) leave their homes, they are often exposed to more victimization and discrimination than their non-SGM peers, including violence and abuse faced in homeless shelters. Furthermore, rates between 5%-48% of youth who experience homelessness suffer from posttraumatic stress disorder. Homeless veterans have​ had a significant impact on mental health in the U.S. Increased levels of suicidal thoughts, uneasiness, and depression have been noted in multiple investigations. Veterans who were dealing with psychotic disorders (PSY) and veterans who recently experienced homelessness (RHV) all showed increasing levels of loneliness, and resulted in demonstration of decrease in social functioning. Furthermore, lower social support results in higher urban stress and lower chance of psychological symptoms. Therefore, less social support can be linked to the detrimental effects of urban stress on psychiatric symptoms. The Open Dialogue Approach, which de-emphasizes pharmaceutical intervention and instead establishes a dialogue with the patient, has been found to be effective in reducing hospitalization rates, medication use, and long-term disability, as well as improving patient outcomes and satisfaction.

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Any illness you have or will have might be caused by an entity you’ll never see. My project will cover how Jinn possession and other Islamic beliefs can affect whether or not a Muslim will receive proper care for their mental health issues. This research was done by analyzing 10 different scholarly sources and their results. While many Muslims believe in Jinn, there is a minority of Muslims who believe that Jinn caused their mental illness, of which hallucinations, hearing voices, and paranoia are one of the few symptoms that are heavily attributed to Jinns. Because of evil Jinns, not many are comfortable talking about their problems due to possibly inviting more evil omens. These beliefs have given a negative stigma towards mental illness causing issues like harassment and shame, leading many to not seek out professional help for fear of judgment. In addition, there has been a trend of Muslims going to faith healers to get care instead of relying on healthcare professionals due to mistrust and lack of education. This can cause many complications, one major being that mentally ill patients might not get diagnosed properly and might even get poor treatment. Other minor factors include income levels and societal/family pressure, which might affect a Muslim's decision. Better education on mental illness from religious leaders and learning to trust professionals, along with professionals understanding these religious beliefs, are essential to lessen the stigma of mental illness and allow proper diagnoses and help.

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It is a given fact that everyone will age as time passes so it is important to be aware of the impact getting older will have on our brains to keep it as healthy and functional as possible. This study aims to distinguish the effects of age on a human's neural processing ability and neuroplasticity, and how this affects their ability to acquire information and respond to emotional situations in the world/people around them. To piece together a conclusion, 5 peer reviewed studies and sources were analyzed. It was found through this analysis that a person's ability to learn and regulate emotions resembles a bell curve, meaning it gradually improves from birth to physical peak, then once again starts to decline. This doesn't mean that hope is lost after a certain age. It was also found that people can make conscious efforts to sustain their neuroplasticity to skew the bell curve of their brains’ abilities, keeping it agile including learning ability and regulating emotions. This included doing things such as playing mentally stimulating games ie. chess and sudoku to ‘work out’ the mind similarly to how physical exercise improves health of muscles in the body. The implications of these findings aid people in retaining mental abilities with aging. People commonly fear aging due to the toll it can have on mental stability/agility. Utilizing this information can help put minds at ease, knowing there are steps that can be taken to prolong these negative side effects.

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We are conducting a survey research study to investigate whether mood has an effect on one's stress level and critical thinking abilities. This research topic is important because critical thinking is an important ability, especially for college students, and can be influenced by mood and stress. Because increased stress levels are prevalent among college students and have a variety of negative impacts on both physical and mental health, researching the relationship between stress, mood, and critical thinking can lead to a better understanding of critical thinking processes. Through better understanding of mood and stress, learning environments for college students could be improved to better enhance critical thinking abilities. 

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This project will look at the various types of news platforms used to consume news in relationship with overall mood. A survey will gather demographic information and the types of various platforms used. A video will be assigned to a participant with a news clip. The news clip will be about the same current event, but with different emotionally charged content. The independent variable will be either a negatively charged news clip, a positively charged news clip, and for a controlled group it will be a weather report. After viewing the video, the participant will take a survey that asks questions on a rating scale relating to depression and anxiety. After data is compiled we will look for any trends with the type of news content consumed and levels of depression and anxiety about the world around us. 

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The impact of postmodernism on the media in the United States has resulted in a shift away from traditional notions of objectivity in journalism and a reconsideration of the role of the state in regulating the media, leading to a more diverse and pluralistic media landscape. This new media landscape has changed the role of the United States’ media landscape quite significantly, most notably with the profound spread of social media. Historically, there have been actions taken to ensure media rights, as well as restrictions on what these rights actually protect, which has changed the role of the United States’ media landscape. While the right to free media remains a crucial component of modern society, the evolution of media and its impact on society calls for the implementation of appropriate regulations, highlighting the need for media regulation in the postmodern era.

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The Pygmalion effect describes the reinforcing feedback loop of student success, caused by positive or negative expectations set by teachers. The effect is a significant predictor of academic achievement in students based on teacher presuppositions of them. Generally, if expectations are high, academic results will be high and vice versa.

Academic achievement is statistically significantly different between students at schools with higher expectations versus those at schools with lowered expectations (Dobbie and Fryer). This creates discrepancy in the learning achieved by different students.

This inconsistency is caused by teachers forming judgements based on factors including socioeconomic status, ethnicity, and gender. Methods usually synthesized to shrink this difference include instructing teachers to implement high expectation behaviors, making teachers aware of the effects of expectations and biased expectations, and addressing the beliefs of teachers that influence biased expectations (de Boer, Timmermans, and van der Werf).

While the relative effectiveness of each method may vary, employing any of these methods produces a net positive effect on both increasing teacher expectations and student achievement. Furthermore, additional future research on the third method, addressing underlying beliefs of teachers that form the basis of assumptions, is needed to confirm or refute its potential of being the most effective approach to maximizing the benefits of the Pygmalion effect. 

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I will try to cover Confucius and his moral philosophy. I hope to apply how influential his way of thinking was, not that others take use him as an example but his knowledge and application of it was groundbreaking. I also want to look at ancient Greek philosophers, and the start of political science with Aristotle. 

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