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Part Three (3): IMMORTALITY; Chapters 23-27
Part Three (3): IMMORTALITY; Chapters 23-27
Chapter Lectures and Annotation Supports
Chapter Lectures and Annotation Supports
Part Three (3): IMMORTALITY
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Chapter 23: "It's Alive" 1973-74
Chapter 24: "Least They Can Do" 1975
Chapter 25: "Who Told You You Could Sell My Spleen?" 1976-88
Chapter 26: Breach of Privacy 1980-85
Chapter 27: The Secret of Immortality 1984-95
Session Four (4): Weekly Agenda
Culture Clash: Cell Cultures vs. the "Golden Age of Antibiotics"
Culture Clash: Cell Cultures vs. the "Golden Age of Antibiotics"
The "Golden Age of Antibiotics"
The "Golden Age of Antibiotics"
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- Molds, spores, fungi, & plant extracts used for millennia (Egypt)
- Paul Ehrlich (1909), Germany. Arsphenamine on syphilis
- Alexander Fleming (1928), Scotland. Penicillium/Staphylococcus
- Howard Florey & Ernst Chain (1945), Oxford. Mass production
- Evolution through Natural Selection on the micro-scale!
- competition for resources (balance of spread & infection)
- biodiversity increases through differential reproduction, heredity, and variation
FieldDay: The Yard (2020). Antibiotic Resistance: An Interactive Explorable. Retrieved from https://www.brainpop.com/games/antibiotic resistancegame/ on 1 May 2020.
Labster (2020). Thermofisher: Cell Culture Basics. Retrieved from https://www.thermofisher.com/us/en/home/global/forms/cell-culture-basics/cell-culture-basics-virtual-lab.html on 1 May 2020.
Magdalena Jedrzejczak-Silicka (May 10th 2017). History of Cell Culture, New Insights into Cell Culture Technology, Sivakumar Joghi Thatha Gowder, IntechOpen, DOI: 10.5772/66905. Available from: https://www.intechopen.com/books/new-insights-into-cell-culture-technology/history-of-cell-culture
Helpful HIPPA Hints & Gel Electrophoresis Tips
Helpful HIPPA Hints & Gel Electrophoresis Tips
Gel Electrophoresis
Gel Electrophoresis
"Every individual’s DNA is unique, with the only exception being identical twins. It is because of this fact that DNA is so valuable in many criminal investigations. A suspect can be identified using his or her DNA profile. In 1984, a British scientist name Alec Jeffreys developed a technique utilizing the variation in DNA sequences to identify individuals. Restriction endonucleases (commonly called restriction enzymes) act as molecular scissors that can cut DNA in specific location. Because every individual’s DNA is slightly different, an individual’s code determines the number of times the restriction enzymes will cut and the number and size of DNA pieces that will result. These pieces can then be separated and compared using a process called gel electrophoresis. As these fragments move, their varying lengths propel them through an agarose gel at different speeds. Scientists can use these RFLPs, Restriction Fragment Length Polymorphisms, a set of DNA puzzle pieces unique to the individual, to create a pattern called a DNA fingerprint. In order to avoid the confusion with actual fingerprinting, this technique is now often called DNA profiling. There are many processes now used in DNA profiling and they are used in forensic and legal cases where relatedness or identify is in question. In order to perform DNA analysis, large amounts of DNA need to be present. This is possible through a process called Polymerase Chain Reaction (PCR). PCR enables scientists to produce millions of copies of a specific DNA sequence from a small amount of DNA, such as that left at the scene of a crime" (PLTW, 2013).
Project Lead the Way (PLTW) (2013). Activity 1.2.3: DNA Analysis. Retrieved from https://docs.google.com/document/d/1EpBPxvsJR5KqeU mn6s3OUIE5LiGyKyNdKm5AsFQ6CyQ/edit?usp=sharing on 1 May 2020.
Project Lead the Way (PLTW) (2015). Activity 1.3.2: Confidentiality. Retrieved from https://docs.google.com/presentation/d/1dlzw5MHaitbO 1cji8KaGdPRl31cfZBD0p-7pScmwOnk/present?slide=id.p4 on 1 May 2020.
The Fountain of Youth?
The Fountain of Youth?
In Chapter 27, The Secret of Immortality, Skloot describes the excitement that many scientists felt as the searched for answers to decreasing cell mortality rates and increase human life expectancy.
The telomere, or "cap" to your chromosomes, play vital roles in the replication, longevity, and separation of your genetic material!
Replication: most cells can grow and divide upwards of 50 times
Longevity: notice the repeating nucleotide subunit on the end
Separation: proteins prevent chromosomes from joining in M1/M2
R&D Systems, Inc. (2020). Telomerase. Retrieved from https://www.rndsystems.com/resources/articles/telomerase on 1 May 2020.
Skloot, R. (2010). The Immortal Life of Henrietta Lacks; Chapter 27: The Secret to Immortality. New York, New York; Crown Publishers.
Understanding the COVID-19 Pandemic
Understanding the COVID-19 Pandemic
Relevant Applications with COVID-19/Coronavirus
Understanding the COVID-19 Pandemic:
Understanding the COVID-19 Pandemic:
Insights from Johns Hopkins University Experts
Johns Hopkins University & Medicine (2020): Understanding the COVID-19 Pandemic: Insights from Johns Hopkins University Experts. Retrieved from https://coronavirus.jhu.edu/covid-19-basics/understanding-covid-19 on Friday, 1 May 2020.
Johns Hopkins University & Medicine (2020): COVID-19 Dashboard by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (JHU). Retrieved from https://coronavirus.jhu.edu/map.html on Friday, 1 May 2020.
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