Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Complete Models
However, we will continue to brainstorm, sketch, Solidworks, 3D print, and test
Completed models : 24 hpf dorsal up Transplantation mold (T-mold) iteration 1 (I1)/ I2/I3/I4, 48 hpf dorsal up T-mold I1/I2, 24 hpf side T-mold I1, 24hpf dorsal up imaging mold (I-mold) I1, 24 hpf ventral up T-mold I1, 24hpf dorsal up drug mold
Completed Solidworks: 24 hpf dorsal up direct mold (D-mold) I1
Accomplishments from Previous Week
Research microfluidics techniques
Print microfluidics drug testing mold
Goals for Next Week (list names after each item). Use specific and measurable objectives.
First draft of presentation/poster (Connie & Amanda)
Finalize designs with Dr.Wang (Connie)
Print 5 of each mold (Connie)
Test microfluidics mold prototype with Dr.Wang (Amanda)
Place purchase for Stratasys material (Amanda)
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Will test 48 hpf molds this week
Dr.Klemke would like to look into a way to separate cell nucleus from cytoplasm
Send information about poster session date and time
Instructor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Can still look into direct mold with off campus printing
Don’t think it’s an economical choice because agarose molds are so cheap
Risks and Areas of Concern
Still no major concerns as of now
Resources or Information Required but not Available
Specific fish dimensions (websites may not be super accurate)
Have determined that this is not necessary as the molds work rather well with approximate dimensions
Plastic toxicity to fish (not much research conducted on this)
May have to experiment with different materials
Have research on the toxicity of the liquid material but not the UV cured
Schedule
See updated Gantt chart.
Budget (list amount spent and amount remaining)
Amount Spent
First mold for transplantation: $12 (3/11/16)
Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)
Third Iteration 24hpf dorsal up, 3 Imaging molds for 24hpf, 24hpf side orientation, 48hpf side orientation: $27.62 (4/25/16)
5x 24hpf Side I2: $43.59 (5/3/16)
Over 3D printing budget of $100: Purchase order of $250 for Stratsys
24 hpf Dorsal I4, 24 hpf Ventral I1: $22 (5/12/16)
24 hpf Dorsal Drug I1: $TBD
Amount Remaining: $1144.79
Progress on Report and Webpage
Report mostly complete waiting to add finalized design specifications
Internal Pages of webpage have been updated up to week 9
Exteral webpages are complete except for finalized design specifications