Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Solidworks modeling under way
Will be a continuous process between brainstorm, sketch, Solidworks, 3D print, and test
Completed Solidworks models : 24 hpf dorsal up T-mold iteration 1/iteration 2, 48 hpf dorsal up T-mold iteration1, 24 hpf side up T-mold iteration 1, 24 hpf dorsal up direct mold iteration 1.
First fabrication printed and tested
The transplantation mold for 24 hpf with dorsal up
Holds embryos well but tail portion needs to be made deeper
First fabrication of 48 hpf printed
Transplantation mold dorsal up
Second iteration of transplantation mold for 24 hpf dorsal up printed
Accomplishments from Previous Week
CAD for transplantation mold for 24 hpf side orientation
CAD for direct holding mold for 24 hpf dorsal up
Goals for Next Week (list names after each item). Use specific and measurable objectives.
3D print of direct holding mold (Connie & Amanda)
Test direct holding mold (Amanda)
CAD of design for 48 hpf of side orientations (Connie)
3D print side orientation molds (Connie & Amanda)
Test side orientation molds (Amanda)
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Notes from last time because haven’t met with sponsors since last web update (meeting Thursday):
First prototype works! However can be improved if the tail region was deeper
Tail still moves a bit and microinjection isn’t as accurate
But still much more efficient than previous methods!
Created a second iteration and have sent it for testing
Have side orientation and 48 hpf soon
Have printed the 48hpf and sent for testing
Side orientation is ready for printing
Make a small scale one that produces agarose mold that cuts all the way through to glass for imaging purposes
Started on CAD for this design
Mineral oil residue leaves agarose greasy; find a way to get rid of the oil - ethanol doesn’t work
Doesn’t seem to have any impact on the fish development though
Currently testing molds without post processing to avoid the use of mineral oil
Instructor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Molds do not need to post processed if you don’t mind the wax on it (during presentation 4/6)
Comments from Other Students in the Class (indicate date of comments and if via email or in person)
Have not received presentation feedback
Risks and Areas of Concern
No major concerns as of now
Resources or Information Required but not Available
Specific fish dimensions (websites may not be super accurate)
May need to get measurements by examination
Since current molds work without exact measurements we will only get the dimensions if it is critical
Plastic toxicity to fish (not much research conducted on this)
May have to experiment with different materials
Have research on the toxicity of the liquid material but not the UV cured
Schedule
See updated Gantt Chart
Budget (list amount spent and amount remaining)
Amount Spent
First mold for transplantation: $12 (3/11/16)
Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)
Amount Remaining: $1466
Progress on Report and Webpage
Select sections of the report started
Background, current solutions, requirements, deliverables, title page, appendix
Internal pages of webpage have been updated
Week 3 being worked on