Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Complete Models
However, we will continue to brainstorm, sketch, Solidworks, 3D print, and test
Completed models : 24 hpf dorsal up Transplantation mold (T-mold) iteration 1 (I1)/ I2/I3/I4, 48 hpf dorsal up T-mold I1/I2, 48 hpf side up T-mold I1, 48 hpf ventral T-mold I1 24 hpf side T-mold I1, 24hpf dorsal up imaging mold (I-mold) I1, 24 hpf ventral T-mold I1, 24hpf dorsal up drug mold
Completed Solidworks: 24 hpf dorsal up direct mold (D-mold) I1
Accomplishments from Previous Week
Finalized all 24hpf mold designs
Final iteration of 48hpf mold design
Printed 24hpf drug test mold design
Goals for Next Week (list names after each item). Use specific and measurable objectives.
Final draft of presentation/poster (Connie & Amanda)
Finalize 48hpf designs with Dr.Wang (Connie)
Print 5 of each 48hpf mold (Connie)
Test microfluidics mold prototype with Dr.Wang (Amanda)
Place purchase for Stratasys material (Amanda)
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Will test 48 hpf molds this week
Needs the tail area to be longer and overall should be deeper
Dr.Klemke would like to look into a way to separate cell nucleus from cytoplasm
Not enough time to really work on this, will have to be another project in the future
Send information about poster session date and time
Emailed details and will be doing a run of the presentation at sponsor meeting
Instructor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
None
Comments from Other Students in the Class (indicate date of comments and if via email or in person)
How many embryos per mold?
What is the life span of the mold?
Explain microinjection process in abstract
Have one drawing per page of the Solidworks models in the Appendix
Don't use the work cheap
Make sure everything is past tense
Risks and Areas of Concern
Still no major concerns as of now
Resources or Information Required but not Available
Specific fish dimensions (websites may not be super accurate)
Have determined that this is not necessary as the molds work rather well with approximate dimensions
Plastic toxicity to fish (not much research conducted on this)
May have to experiment with different materials
Have research on the toxicity of the liquid material but not the UV cured
Schedule
See updated Gantt Chart
Budget (list amount spent and amount remaining)
Amount Spent
First mold for transplantation: $12 (3/11/16)
Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)
Third Iteration 24hpf dorsal up, 3 Imaging molds for 24hpf, 24hpf side orientation, 48hpf side orientation: $27.62 (4/25/16)
5x 24hpf Side I2: $43.59 (5/3/16)
Over 3D printing budget of $100: Purchase order of $250 for Stratsys
24 hpf Dorsal I4, 24 hpf Ventral I1: $22 (5/12/16)
24 hpf Dorsal Drug I1, 3x 24hpf Dorsal 14, 3x 24hpf Ventral I1: $53.56
1x 24hpf Dorsal 14, 1x 24hpf Ventral I1, 48hpf Dorsal I2, 48hpf side I1, 48hpf ventral I1: $TBD
Amount Remaining: $1144.79
Progress on Report and Webpage
Report mostly complete waiting to add finalized design specifications
Internal Pages of webpage have been updated up to week 10
Exteral webpages are complete except for finalized design specifications