156B Week 6

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Complete Models

    • However, we will continue to brainstorm, sketch, Solidworks, 3D print, and test

    • Completed Solidworks models : 24 hpf dorsal up Transplantation mold (T-mold) iteration 1 (I1)/ I2/I3, 48 hpf dorsal up T-mold I1/I2, 24 hpf side T-mold I1, 24 hpf dorsal up direct mold (D-mold) I1, 24hpf dorsal up imaging mold (I-mold) I1.

Accomplishments from Previous Week

• • Printed 5 24hpf Side T-molds per Dr.Wang’s request

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Revise models based on feedback and print new models (Connie)

  • Test the new transplantation molds with Dr.Wang and gather feedback (Amanda)

  • Request material ordering information from Stratysys and submit purchase forms (Amanda)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Make spacing between each well closer (condense the pattern)

  • 48hpf iteration 1 mold has too small of yolk sac area and too large of head area

  • 24hpf side I1 is very good and will need 5 copies of it to make multiple agarose molds

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Ask sponsors about anything else we can do

    • Sponsors responded with general off the top of their head ideas like cell picking and sonication

    • Indicated they would discuss more later on

Risks and Areas of Concern

• • Still no major concerns as of now

Resources or Information Required but not Available

• • Specific fish dimensions (websites may not be super accurate)

    • May need to get measurements by examination

  • Plastic toxicity to fish (not much research conducted on this)

    • May have to experiment with different materials

    • Have research on the toxicity of the liquid material but not the UV cured

Schedule

• • See updated Gantt chart.

Budget (list amount spent and amount remaining)

• • Amount Spent

    • First mold for transplantation: $12 (3/11/16)

    • Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)

    • Third Iteration 24hpf dorsal up, 3 Imaging molds for 24hpf, 24hpf side orientation, 48hpf side orientation: $27.62 (4/25/16)

    • 5x 24hpf Side I2: $43.59 (5/3/16)

  • Amount Remaining: $1394.79

Progress on Report and Webpage

• • Project background, review of existing solutions, requirements, deliverables, and impact on society sections of final report are completed.

  • Rough draft of final report completed

  • Internal Pages of webpage have been updated up to week 6

  • External Pages are being updated with “Team” and “Budget” complete (continuous updates)

    • “Reports” is continually being added to

    • “Home” is continually being added to