This project required a mold that holds multiple zebrafish embryos in the proper orientation. The embryos must be held stationary in order for the injection process to achieve precision in the positioning of the injection sites.
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The final design consisted of a transplantation mold that has raised cutouts in the shape of the embryos on the surface. The mold is then placed face down inside a petri dish filled with liquid agarose. The agarose then solidifies and takes the shape of the embryos. The mold is then removed leaving behind embryo shaped crevices in the agarose.