Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Solidworks modeling under way
Will be a continuous process between brainstorm, sketch, Solidworks, 3D print, and test
First fabrication printed and tested
The transplantation mold for 24 hpf with dorsal up
Holds embryos well but tail portion needs to be made deeper
First fabrication of 48 hpf printed
Transplantation mold dorsal up
Accomplishments from Previous Week
Printed second iteration for 24 hpf dorsal up transplantation mold
Printed first iteration for 48 hpf dorsal up transplantation mold
Tested both molds in lab
Goals for Next Week (list names after each item). Use specific and measurable objectives.
CAD for transplantation mold and 24hpf side orientation (Connie)
Print mold for both design (Connie & Amanda)
Test design in lab (Amanda)
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
First prototype works! However can be improved if the tail region was deeper
Tail still moves a bit and microinjection isn’t as accurate
But still much more efficient than previous methods!
Have side orientation and 48 hpf soon
Make a small scale one that produces agarose mold that cuts all the way through to glass for imaging purposes
Mineral oil residue leaves agarose greasy; find a way to get rid of the oil - ethanol doesn’t work
Doesn’t seem to have any impact on the fish development though
Instructor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
Be more specific in describing actions done in progress report
Look for any possible soft glass for both injection and imaging process
Brought up the idea of using silicon instead of glass
Or maybe acrylic (should ask Tom)
Comments from Other Students in the Class (indicate date of comments and if via email or in person)
Make more eye contact during presentation
Split talking more evenly
Make sure to explain terminology
Good job on answering any clarifying questions
Clear presentation and concise slides
Risks and Areas of Concern
No major concerns as of now
Resources or Information Required but not Available
Specific fish dimensions (websites may not be super accurate)
May need to get measurements by examination
Plastic toxicity to fish (not much research conducted on this)
May have to experiment with different materials
Schedule
See updated Gantt chart.
Budget (list amount spent and amount remaining)
Amount Spent
First mold for transplantation: $12 (3/11/16)
Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)
Amount Remaining: $1466
Progress on Report and Webpage
Report outline has begun
Internal pages of webpage have been updated
Week 2 being worked on