156B Week 4

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Solidworks modeling complete

    • However, we continue to brainstorm, sketch, Solidworks, 3D print, and test

    • Completed Solidworks models : 24 hpf dorsal up T-mold iteration 1/iteration 2, 48 hpf dorsal up T-mold iteration1, 24 hpf side up T-mold iteration 1, 24 hpf dorsal up direct mold iteration 1.

  • First fabrication printed and tested

    • The transplantation mold for 24 hpf with dorsal up

    • Holds embryos well but tail portion needs to be made deeper

  • First fabrication of 48 hpf printed

    • Transplantation mold dorsal up

    • Currently being tested in lab

  • Second iteration of transplantation mold for 24 hpf dorsal up printed

  • Currently being tested in lab

Accomplishments from Previous Week

• • CAD for transplantation mold for 24 hpf imaging

• CAD for transplantation mold for 48 hpf side orientation

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Send all new CADs of transplantation molds to 3D Print (Connie)

  • CAD of design for direct holding mold for compatibility with Connex3 (Connie)

  • Test rest of the transplantation molds molds (Amanda)

• Research cost and Connex3 Printer biocompatibility (Amanda)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)

• • Transplantation molds with the wax on (no post processing) still create agarose molds just fine

  • Make a small scale one that produces agarose mold that cuts all the way through to glass for imaging purposes

  • Have side orientation and 48 hpf soon

    • All of these have been sent for 3D printing

Instructor Comments from Last Meeting and Actions Taken to Address these Comments  (indicate date of comments and if via email or in person)

• • Remember to put a taper on the mold design for easy release (~2 degrees)

  • Bring agarose mold to next meeting

• Add cancer cell photos to presentations

Comments from Other Students in the Class (indicate date of comments and if via email or in person)

• • Clear slides, good documentation, prototype was helpful in understanding project

  • Clarify material selection process, speak slower and clearer, condense text in some slides (too wordy)

Risks and Areas of Concern

• Still no major concerns as of now

Resources or Information Required but not Available

• • Specific fish dimensions (websites may not be super accurate)

    • No longer pertinent as mold works without specific measurements

  • Plastic toxicity to fish (not much research conducted on this)

    • Current biocompatible material has not shown any impact on embryo development

  • Have research on the toxicity of the liquid material but not the UV cured

Schedule

• • See updated Gantt Chart

Budget (list amount spent and amount remaining)

• • Amount Spent

    • First mold for transplantation: $12 (3/11/16)

    • Second iteration for 24 hpf dorsal up orientation and first iteration for 48 hpf dorsal up: $22 (4/5/16)

    • Waiting for approximation of material weight tomorrow morning (4/20)

• Amount Remaining: $1466 (changes will be reflected when weight of material is known)

Progress on Report and Webpage

• • Project background, review of existing solutions, requirements, deliverables, and impact on society sections of final report are completed.

  • Internal pages of webpage have been updated

  • Week 4 being worked on