AP Bio

Every year, the AP Biology students get the opportunity to go to Cold Spring Harbor Lab to apply their learned knowledge to real-life scenarios. This February 5th and March 13th, both AP classes took the exciting annual field trip and experienced what it is like to work in a lab and participate in hands-on scientific techniques. The trip focused on two molecular biological techniques: polymerase chain reaction (PCR) and gel electrophoresis.

Before the visit, students learned about the processes of both techniques in the curriculum during the genetics and biotechnology unit. However, during the lab, professionals went more in-depth and showed the students machines/tools, the methodologies, and how to prepare the samples. PCR amplifies DNA samples or creates more of it for testing purposes. For PCR, students were instructed to rub a cotton swab on the inside of their mouth to collect cheek cells. We used devices such as centrifuges and micropipettes. Our DNA samples were heated in a machine to denature the samples before the rest of the PCR process could continue. While waiting for the machine to finish, the students and advisors took a lunch break where they chatted in anticipation of the next activity. They were also permitted to walk around and browse the exhibits. 

Returning from the lunch break, the students moved on to gel electrophoresis. This laboratory method separates DNA fragments based on size. The classes carefully placed their samples into a gel and watched as the genetic material separated and moved through the substance, displaying bands of different DNA sizes. Although some didn't work as expected, the professionals promised to redo the experiment to get accurate results.

After the field trip, students were assigned a lab activity, where they were able to compare their own DNA sequences to those of other students in their class, animals, and even ancient human beings. Such comparisons revealed genetic similarity, essentially, how closely related we are to one another and to ancient beings. 

The field trip to Cold Spring Harbor Lab was an insightful and engaging experience that developed students’ understanding of biotechnology and how scientists study DNA, as well as inspiring those who are contemplating a career in science.