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Optimum pH is the pH at which the enzyme is the most active.
Optimum pH is the pH at which the enzyme is the most active.
The data provided for each enzyme were used to prepare graphs (Product vs. pH) shown at the bottom of the page. Use these data and graphs to determine the Optimum pH for each enzyme.
The data provided for each enzyme were used to prepare graphs (Product vs. pH) shown at the bottom of the page. Use these data and graphs to determine the Optimum pH for each enzyme.
Reaction Conditions
Reaction Conditions
The following pH values were tested for each enzyme:
The following pH values were tested for each enzyme:
pH 1.00
pH 2.30
pH 3.60
pH 4.90
pH 6.20
pH 7.50
pH 8.80
pH 10.10
pH 11.40
pH 12.70
pH 14.00
Shown below are the incubation conditions that were used for these experiments:
Shown below are the incubation conditions that were used for these experiments:
Incubation Time 10 minutes
Amount of Enzyme 100 ul
Temperature 30 degrees Celsius
Substrate Concentration 100 mM
Results
Results
Select an enzyme to see the results of varying the pH on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Select an enzyme to see the results of varying the pH on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Enzyme 1
Enzyme 1
pH product (µmol)
1 0
2.3 1.15
3.6 32.45
4.9 65.71
6.2 9.54
7.5 0.1
8.8 0
10.1 0
11.4 0
12.7 0
14 0
Enzyme 2
Enzyme 2
pH product (µmol)
1 0
2.3 0
3.6 0
4.9 0.02
6.2 1.15
7.5 12.35
8.8 25.4
10.1 9.57
11.4 0.68
12.7 0.01
14 0
Enzyme 3
Enzyme 3
pH product
1 0
2.3 0
3.6 0.05
4.9 2.98
6.2 21.88
7.5 20.21
8.8 2.33
10.1 0.03
11.4 0
12.7 0
14 0
Enzyme 4
Enzyme 4
pH product
1 0
2.3 0
3.6 0
4.9 0
6.2 0
7.5 4.35
8.8 24.04
10.1 9.49
11.4 0.27
12.7 0
14 0
Enzyme 5
Enzyme 5
pH product
1 6.6
2.3 25.31
3.6 12.26
4.9 0.77
6.2 0.01
7.5 0
8.8 0
10.1 0
11.4 0
12.7 0
14 0
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
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