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Some enzymes form dimers or aggregates at high concentrations. This can increase or decrease activity compared to the monomer.
Some enzymes form dimers or aggregates at high concentrations. This can increase or decrease activity compared to the monomer.
The data provided for each enzyme below was used to prepare a graph of product vs. enzyme amount. Find the region of the graph where the slope is the steepest, this is the range of enzyme concentrations where the enzyme is the most active.
The data provided for each enzyme below was used to prepare a graph of product vs. enzyme amount. Find the region of the graph where the slope is the steepest, this is the range of enzyme concentrations where the enzyme is the most active.
Reaction Conditions
Reaction Conditions
The following amounts of enzyme were tested for each set of experiments
The following amounts of enzyme were tested for each set of experiments
0 ul
25 ul
50 ul
75 ul
100 ul
125 ul
150 ul
175 ul
200 ul
225 ul
250 ul
Shown below are the Incubation Conditions that were used
Shown below are the Incubation Conditions that were used
pH Optimal pH for each enzyme
Incubation Time 10 minutes
Temperature 30 degrees Celsius
Substrate Concentration 100 mM
Enzyme Selection
Enzyme Selection
Select an enzyme to see the results of varying the enzyme amount on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Select an enzyme to see the results of varying the enzyme amount on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Enzyme 1
Enzyme 1
enzyme (µl) product (µmol)
0 0.03
25 14.77
50 29.43
75 43.62
100 56.52
125 68.01
150 79.4
175 88.49
200 92.3
225 96.43
250 99.06
Enzyme 2
Enzyme 2
enzyme (µl) product (µmol)
0 0.01
25 11.04
50 21.78
75 32.38
100 43.12
125 52.61
150 61.76
175 69.87
200 78.31
225 85.74
250 90.76
Enzyme 3
Enzyme 3
enzyme (µl) product (µmol)
0 0.03
25 6.47
50 13
75 19.41
100 25.24
125 30.94
150 37.79
175 42.81
200 48.56
225 54.22
250 58.54
Enzyme 4
Enzyme 4
enzyme (µl) product (µmol)
0 0.02
25 7.24
50 14.07
75 21.59
100 28.47
125 34.64
150 41.89
175 47.46
200 53.84
225 59.04
250 63.72
Enzyme 5
Enzyme 5
enzyme (µl) product (µmol)
0 0.02
25 11.16
50 22.19
75 33.46
100 43.28
125 52.07
150 61.69
175 69.81
200 74.93
225 81.19
250 87.78
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
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